cGHR基因第8及第9内含子的克隆和分析  

Cloning and Analysis of Intron 8 and 9 of Chicken GHR Gene

作　　者：欧阳建华[1] 柳小春[2] 施启顺[2] 孙汉[1] 

机构地区：[1]江西农业大学动物科学技术学院,江西南昌330045 [2]湖南农业大学动物科学技术学院,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2005年第2期195-198,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：江西省自然科学基金资助项目(0130039)

摘　　要：通过克隆测序,获得cGHR基因内含子8及9的全部序列,内含子8长1397bp,内含子9长272bp.为验证该两序列为新发现的未知序列,将该两条序列在NCBI网站上进行Blast分析.结果表明,该两段序列均为新发现的未知序列,GenBank登录号分别为AY481574和AY327492.cGHR基因内含子序列的获得,有利于进一步研究cGHR基因的表达调控和全基因的SNPs筛选.By cloning and sequencing,the whole sequences of 8th and 9th intron of chicken growth hormone receptor (cGHR) gene in lgehorn chicken were obtained,which were accepted by GenBank with the accession number of AY481574 and AY327492 respectively.The discovery could provide basic sequence for later analysis on the expression and single nucleotide polymorphism(SNP)discovering of chicken GHR gene.

关 键 词：cGHR 内含子 克隆 

分 类 号：S828.2[农业科学—畜牧学]