阿司匹林对人巨核细胞体外增殖的影响  

Effect of Aspirin on M egakaryocytes Multiplication in Vitro

作　　者：邵荣姿[1] 马玉桂[2] 王鲁群[2] 

机构地区：[1]解放军济南军区总医院药剂科,济南250031 [2]济南军区总医院血液病科

出　　处：《中国药师》2005年第5期366-368,共3页China Pharmacist

摘　　要：目的:探讨阿司匹林对巨核细胞增殖、膜抗原表达及凋亡的影响。方法:将骨髓单个核细胞接种于含不同浓度阿司匹林的H2000培养基中,其中含Tpo 50ng·ml-1、SCF 50 ng·ml-1和IL-3 10ng·ml-1,5%CO2、37℃、饱和湿度的孵育箱中培养14 d。结果:高浓度阿司匹林使巨核细胞的增殖变化明显高于低浓度,巨核细胞集落(CFU-MK)数减少,细胞丛(FFU-MK)数增加,CD41膜抗原表达率显著降低(P<0.05);AnnexinV表达率明显增高(P<0.05);DNA倍体分析的凋亡指数明显升高(P< 0.05);电镜观察可见出芽、空泡样结构和胞浆内颗粒减少。结论:高浓度阿司匹林可影响巨核细胞的正常代谢和促进其凋亡。Objective: To investigate the effect of aspirin on megakaryocytes multiplication in Vitro. Method: Separated megakaryocytes were cultured in vitro with medium H2000 containing aspirin 0,1.6,3.2,6. 4,12. 8, and 32. 0 mg·L-1 ,of which containing Tpo 50 ng·ml-1,SCF 50 ng·ml-1 and IL-3 10ng·ml-1 at the condition of 5% CO2 ,37 ℃ and saturation humidity for 14 days. Result: The megakaryocytes'multiplicating change produced by the higher concentration of aspirin was significantly higher than that of the lower concentration of aspirin. The mumber of megakaryocytes colony-forming unit was decreased, the flora-forming unit of megakaryocytes was increased,the expression rate of membrane antigen CD41 was significantly decreased(P<0.05); the expression of AnnexinV was significantly increased(P<0.05), the apoptosis index analysed by DNA diploid was significantly increased; the budding, vacuolization, and granules in cytochylema were decreased by the observation of electron microscopy. Conclusion: The higher concentration of aspirin might interfere with the normal metabolism and accelerate apoptosis of megakaryocytes in Vitro.

关 键 词：阿司匹林 巨核细胞 增殖 膜抗原表达 凋亡 

分 类 号：R971.1[医药卫生—药品]