RT-PCR半定量法检测大蒜素对肉仔鸡肝脏HMG-CoAR、ACC和FASmRNA表达的影响  

A Semi-quantitation RT-PCR Method to Examine the Influence of Garlicinon HMG-CoAR,ACC and FAS mRNA Expression in Chicken Livers

作　　者：万家余[1] 高宏伟[1] 王玉平[1] 孟轲音[1] 段铭[1] 何猛[1] 张光圣[1] 

机构地区：[1]吉林大学农学部畜牧兽医学院,长春130062

出　　处：《安徽农业大学学报》2005年第2期150-152,共3页Journal of Anhui Agricultural University

基　　金：吉林省重点科技攻关项目(吉科合字第 20000210 05号)资助。

摘　　要：通过RT PCR半定量法分析大蒜素对肉仔鸡肝脏中与脂类代谢有关的3种酶HMG CoAR、ACC和FASmRNA表达的影响。结果显示,大蒜素抑制了肉仔鸡肝脏中HMG CoAR、ACC和FASmRNA表达,下降率分别为80 8%、74 2%和64 1%。The effects of garlicin on mRNA expression of HMG-CoAR, ACC and FAS,which were related to lipid metabolism in chicken livers were studied by a semi-quantitation RT-PCR method. The results showed that the mRNA expressions of HMG-CoAR, ACC and FAS in chicken livers were inhibited by garlicin,decreased by 80.8%,74.2%, and 64.1% respectively.

关 键 词：肉仔鸡 HMG-CoAR ACC FAS 

分 类 号：S831[农业科学—畜牧学]