百合栽培品种资源的RAPD分析  

RAPD analysis of Lilium cultivar resources

作　　者：赵庆芳[1] 马世荣[1] 曾小英[1] 李巧峡[1] 崔艳[1] 

机构地区：[1]西北师范大学生命科学学院,甘肃兰州730070

出　　处：《兰州大学学报（自然科学版）》2005年第2期30-33,共4页Journal of Lanzhou University(Natural Sciences)

基　　金：甘肃省计划委员会基金(5002/126)国家星火计划(2001EA86005)资助项目.

摘　　要：基于随机扩增多态DNA(RADP)方法分析了来自3个百合品系的15个栽培百合品种的遗传多样性和亲缘关系.从156个引物中筛选出15个引物,在检测的218个位点中,216个位点为遗传多态位点(99.08%).聚类分析结果表明:来自3个品系的百合栽培品种在遗传关系上明显地聚为3类;亚洲百合品系和东方百合品系的品种有较近的亲缘关系,二者和铁炮百合品系的品种亲缘关系较远;在同一品系内部,相同花色的百合品种具有较近的亲缘关系.The genetic diversity of 15 Lilium cultivars and their relationships were investigated using RAPD markers. Out of 156 oligonucleotidc primers initially screened, 15 were chosen that together generated 218 reproducible bands, of which 216 (PPB 99.8%) were polymorphic. The results showed obvious differentiation between different cultivars strains. Cluster analysis indicated that all the cultivars could be grouped into three clades and the Asiatic Hybrids is more familiar to the oriental Hybrids than the Longiflorum Hybrids. Moreover, the cultivars that have the similar flower color in the same hybrid strain are more familiar than others.

关 键 词：百合 栽培品种 随机扩增的DNA多态性 

分 类 号：Q75[生物学—分子生物学]