双酚A对人胚肝细胞L-02的毒性作用研究  

The Toxic Effects of Bisphenol A on Human Embryo Liver L-02 Cells

作　　者：李华文[1] 刘燕群[1] 石丹[1] 张江华[1] 郝巧玲[1] 吕斌[1] 周宜开[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院环境医学研究所,湖北武汉430030

出　　处：《环境与职业医学》2005年第2期102-104,108,共4页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金资助项目 (编号 :30 4 71 4 33)

摘　　要：[目的]探讨双酚A对肝细胞L- 0 2的毒性作用及可能损伤机制。[方法] 1、10、5 0、10 0 μmol/L浓度的双酚A作用肝细胞L 0 2后,检测双酚A对肝细胞L-0 2的存活率、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、过氧化氢酶(CAT)、丙二醛(MDA)含量变化、细胞凋亡及凋亡相关基因P5 3、caspase3mRNA表达的影响。[结果]双酚A处理后,肝细胞L 0 2的存活率随着双酚A浓度的增加而下降,在≥5 0 μmol/L浓度,细胞的存活率显著降低(P <0 .0 1)。双酚A诱导肝细胞L -0 2中的SOD含量降低(P <0 .0 1) ,GSH和MDA含量在1μmol/L明显降低(P <0 .0 5 ) ,而后随着剂量的增加升高明显(P <0 0 1)。CAT水平先随着剂量的增加而升高到10 μmol/L的(10 5 .68±9.63 )U/g蛋白,而后随着剂量的增加而显著降低(P<0 .0 1)。≥5 0 μmol/L浓度的双酚A能明显诱导肝细胞L 0 2凋亡和P5 3、caspase3mRNA的表达升高(P <0 .0 1)。凋亡率从对照组的(8.16±0 .0 7) %上升到10 0 μmol/L的(17.2 0±1.2 5 ) % ,5 0、10 0 μmol/L浓度P5 3和caspase3mRNA表达分别是对照组的(1.42±0 .2 5 )、(1.65±0 .13 )、(1.41±0 .12 )和(1.77±0 .0 6)倍。[结论]双酚A对肝细胞L 0 2具有细胞毒性和氧化损伤作用,并能诱导肝细胞L 0 2发生凋亡和P5 3。Objective] To study the toxic effects of bisphenol A on human embryo liver L-02 cells and the possible mechanisms. [Methods] Human embryo liver L-02 cells were exposed to different concentration of bisphenol A (1 μmol/L,10 μmol/L,50 μmol/L,100 μmol/L).Survival rate,level of SOD,CAT,GSH,MDA,apoptosis rate and the expression level of p53,caspase3 were measured. [Results] After exposed to bisphenol A,L-02 cell survival rate decreased when the concentration of bisphenol A increased.Bisphenol A significantly decreased SOD level,GSH,MDA level decreased at 1 μmol/L bisphenol A,and then increased as the concentration of bisphenol A increased,respectively.CAT level increased below 10 μmol/L bisphenol A,then significantly increased as the concentration of bisphenol A increased.Apoptosis and expression level of p53,caspase3 mRNA significantly increased at 50 μmol/L and 100 μmol/L bisphenol A,respectively.Apoptosis rate increased from(8.16±0.07)% to(17.20±1.25)% after treatment for 24 h with bisphenol A of varying concentration ranging 0 to 100 μmol/L.Expression level of p53 and caspase3 mRNA increased by 1.42±0.25,1.65±0.13 and 1.41±0.12,1.77±0.06 fold respectively compared with basal level at 50 μmol/L and 100 μmol/L bisphenol A. [Conclusion] Bisphenol A has cytotoxic effect and oxidative damage to L-02 cells,and can induce L-02 cells apoptosis and increase expression level of p53,caspase3 mRNA.

关 键 词：L-02 双酚A 人胚肝细胞 作用研究 超氧化物歧化酶(SOD) caspase3 mol/L 丙二醛(MDA) mRNA表达 氧化损伤作用 过氧化氢酶 基因P53 MDA含量 存活率 损伤机制 毒性作用 谷胱甘肽 含量变化 凋亡相关 细胞凋亡 细胞毒性 对照组 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R994.6[医药卫生—基础医学]