甲醛、三氯化铝、三氯乙烯混合物对吸入染毒小鼠的DNA损伤  

Study on Effects of Mixture(Formaldehyde,Trichloroethylene and Alchlor)Inhalation on DNA Damage in Peripheral Blood Cells of Mice

作　　者：王波[1] 唐萌[1] 王晓娜[1] 谈伟君[1] 

机构地区：[1]东南大学公共卫生学院,南京210009

出　　处：《环境与职业医学》2005年第2期119-121,共3页Journal of Environmental and Occupational Medicine

基　　金：铁道部科技基金资助项目 (编号 :674780 0 30 );江苏省预防医学基金资助项目 (编号 :Y2 0 0 4 2 1 );东南大学科技基金资助项目

摘　　要：[目的]研究甲醛、三氯化铝、三氯乙烯(质量比为6∶11∶3 3 )混合物对小鼠外周血DNA损伤程度及修复情况。[方法]混合物静式吸入染毒,采用单细胞凝胶电泳方法检测不同剂量(4 .12、8.2 5、16.5g/m3 )下不同染毒天数(4、9、11d)小鼠外周血白细胞DNA的损伤情况;检测一次染毒后(3 3g/m3 )不同时间小鼠外周血白细胞DNA的损伤情况。[结果]染毒后小鼠外周血白细胞DNA出现损伤,且损伤程度与染毒次数、染毒剂量具有一定相关性;一次染毒后短期内小鼠即呈现严重的外周血白细胞DNA损伤,72h后基本恢复正常水平。[结论]甲醛、三氯化铝、三氯乙烯(质量比为6∶11∶3 3 )混合物可诱发DNA损伤。Objective] To study effects of mixture(formaldehyde∶trichoroethylene∶alchlor=6∶11∶33)inhalation on DNA damage in peripheral blood cells of mice. [Methods] Static inhalation was applied to expose mice to the mixture and single cell gel electrophoresis(SCGE)assay was used to detect DNA damage in peripheral blood cells of mice at different concentrations(4.12,8.25,16.5 g/m3)and at different exposure time(4 d,9 d,11 d); to detect DNA damages in peripheral blood cells of mice at different time(20 min,3,6,9,12,24,48,72 h)after one exposure at higher level(33 g/m3). [Results] DNA of peripheral blood cells was significantly damaged after exposure,and there are some correlation between exposure time and damage degree,and some correlation between exposure concentration and damage degree; after one exposure the DNA damage was serious within 48 h and recovered after 72 h. [Conclusion] Inhalation of mixture could lead to DNA damage in mice peripheral blood cells.

关 键 词：三氯化铝 三氯乙烯 混合物 甲醛 染毒小鼠 细胞DNA损伤 诱发DNA损伤 外周血DNA 外周血白细胞 损伤程度 损伤情况 鼠外周血 单细胞凝胶 染毒后 吸入染毒 不同剂量 电泳方法 不同时间 染毒次数 染毒剂量 72h后 相关性 量比 

分 类 号：R285.5[医药卫生—中药学] R135.1[医药卫生—中医学]