溴虫腈对小鼠脾、肝、肾细胞DNA的损伤作用  

DNA Damage of Spleen,Liver and Renal Cells of Mice by Chlorfenapyr

作　　者：陈贤均[1] 李小燕[1] 

机构地区：[1]咸宁学院生物学教研室,湖北咸宁437100

出　　处：《环境与职业医学》2005年第2期145-148,共4页Journal of Environmental and Occupational Medicine

摘　　要：[目的]探讨农药溴虫腈对小鼠脾、肝、肾细胞DNA是否有损伤作用及3种器官对溴虫腈的敏感性差异。[方法]溴虫腈按4.9、9.8、19.6mg/kg体重3个剂量,一次性灌胃染毒雄性小鼠,2 4h后用彗星试验检测3种脏器的彗星细胞率和彗星尾长。[结果]各剂量组的彗星细胞率和彗星尾长都显著增加(P <0 .0 5～P <0 .0 0 1) ;脾、肝、肾3种细胞的彗星细胞率及彗星尾长均具有剂量 反应或剂量 效应关系(前者r值依次为0 .9948,0 .9993 ,0 .9890 ,后者r值依次为0 .9999,0 .9999,0 .9898,P值均<0 .0 5 )。比较同剂量组不同器官之间的彗星细胞率,3个剂量组中均为肾细胞>肝细胞>脾细胞(P <0 .0 5～P <0 .0 1) ;比较同剂量组不同器官之间的彗星尾长,3个剂量组均为肾细胞>肝细胞>脾细胞(P<0 0 5 ) ,肝、脾细胞间差异无显著性。[结论]溴虫腈可诱发小鼠脾、肝、肾细胞DNA断裂损伤,肾细胞对溴虫腈致DNA断裂作用最为敏感。Objective] To study whether chlorfenapyr can cause DNA damage of spleen,liver and renal cells in mice and there are differences in the effects of the chlorfenapyr-induced DNA damage among the three kinds of cells. [Methods] Chlorfenapyr was administrated to mice with a dose of 4.9,9.8 or 19.6 mg/kg bw once by gavage,then the percentage of comet cells and the comet tail length of the three kinds of cells were detected by comet assay. [Results] Compared with the corresponding control group,the percentage of comet cells and the comet tail length of the three kinds of cells significantly increased(P<0.05-P<0.001)in the groups treated with chlorfenapyr,in a significant linear dose-dependent manner.Compared at the identical dosage criterion,the percentage of comet cells increased in kidney,liver and spleen cells in descent order,the comet tail length of renal cells,spleen and liver cells increased in descent order(P<0.05)at the three doses of chlorfenapyr,there were no significant differences in the comet tail length between spleen and liver cells. [Conclusion] Chlorfenapyr can induce the DNA damage of spleen,liver and renal cells in mice,and the renal cells are more sensitive to the effects on DNA damage caused by chlorfenapyr than liver cells,and liver cells are more sensitive than spleen cells.

关 键 词：细胞DNA 损伤作用 溴虫腈 剂量-效应关系 DNA断裂损伤 0.05 敏感性差异 剂量-反应 细胞率 肾细胞 雄性小鼠 彗星试验 脾细胞 肝细胞 尾长 器官 一次性 24h 种细胞 显著性 细胞间 r值 

分 类 号：R446[医药卫生—诊断学] R992[医药卫生—临床医学]