新疆盐生植物的钙调蛋白基因克隆与序列分析  

Cloning and Sequence Analysis of the Calmodulin Gene from Halophytes in Xinjiang

作　　者：蔡伦[1] 张富春[1] 曾幼玲[1] 马纪[1] 

机构地区：[1]新疆大学生命科学与技术学院分子生物学重点实验室,新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《植物生理学通讯》2005年第2期163-167,共5页Plant Physiology Communications

基　　金：国家科技攻关西部科技行动项目(2001BA901A32);国家"863"项目(2004AA227110-2)。

摘　　要：采用RT-PCR扩增的方法,从新疆盐生植物花花柴(Karelinia caspica)、盐爪爪(Kalidium foliadum)和盐桦(Betulahalophila)中分别克隆获得了450bp的cDNA片段。基因测序和序列同源性分析的结果表明,所克隆的基因片段均包含了钙调蛋白基因完整的读码框架。新疆花花柴钙调蛋白基因与盐爪爪钙调蛋白基因同源性达86%,盐爪爪与盐桦同源性达86.77%,花花柴与盐桦同源性达85.11%。新疆盐生植物钙调蛋白基因与其它已发表的植物钙调蛋白基因同源性均在80%以上,显示植物钙调蛋白基因具有高度保守性。To clone the calmodulin genes from Karelinia caspica, Kalidium foliadum and Betula halophila in Xinjiang, designed primers were used to amplify 450-bp cDNA fragments with RT-PCR. Sequence analysis revealed that the cloned fragments contained entire calmodulin ORF. Sequence similarity searches were per- formed with BLAST network service.The results showed that high similarity of the calmodulin genes in the three plants, reached 85%. The homology of calmodulin genes between these three plants and the other plants was up to 80%. It indicated that calmodulin gene was highly stable in evolution of plants.

关 键 词：盐生植物 新疆 序列分析 基因克隆 钙调蛋白基因 基因同源性 CDNA片段 PCR扩增 同源性分析 花花柴 基因测序 基因片段 保守性 

分 类 号：Q943.2[生物学—植物学]