变性高效液相色谱法检测鸡HSP70基因单核苷酸多态性  

Single Nucleotide Polymorphisms Detection by Denaturing High Performance Liquid Chromatography in the Chicken HSP70 Gene

作　　者：罗庆斌[1] 郑凤珊[1] 杜红丽[1] 张细权[1] 杨关福[1] 邱祥聘[2] 曾凡同[2] 

机构地区：[1]华南农业大学动物科学学院,广州510642 [2]四川农业大学动物科技学院,四川雅安625014

出　　处：《四川农业大学学报》2005年第1期90-94,共5页Journal of Sichuan Agricultural University

基　　金：国家自然科学基金项目(39970540)。

摘　　要：选取我国20个地方鸡种共591个个体作为实验材料,采用变性高效液相色谱(DHPLC)技术,在鸡HSP70基因的全序列内进行未知SNPs的筛选,对有变异的色谱峰型分别选取2个个体测序,再将同一对引物的所有测序样本的测序结果进行序列的同源性比较,从而识别和确认鸡HSP70基因的未知SNPs。结果分别在4对引物扩增的PCR产物中检出并确认了10个SNPs:A258G、C276G、C507T、C1040A、G1044A、C1431A、T1476C、G1500A、A1529G、C1722T。In this research, 591 individuals from 20 different Chinese native chicken breeds were studied. By using DHPLC technology Unknown SNPs were detected in the chicken HSP70 gene sequence and for every mutant chromatographic types, 2 individuals were selected to sequence. Homologous analysis was carried out based on the sequence information of samples that amplified from same primer to identity new unknown SNPs. In the PCR products amplified by primer A5, A6, A7 and A4, 10 SNPs sites (A258G, C276G, C507T, C1040A, G1044A, C1431A, T1476C, G1500A, A1529G and C1722T) are detected.

关 键 词：变性高效液相色谱 鸡 HSP70基因 单核苷酸多态性 

分 类 号：S831.2[农业科学—畜牧学]