应用聚合酶链式反应(PCR)技术检测野猪的氟烷基因  

Diagnosis of pig's genotyoes for halthane at DNA level by PCR amplification

作　　者：袁子国[1] 耿忠诚[1] 靳锐[1] 迟兆江[2] 李修明[1] 刘丽丽[1] 陈建华[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319 [2]黑龙江省农垦总局科技局,黑龙江哈尔滨150020

出　　处：《黑龙江畜牧兽医》2005年第5期14-16,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：黑龙江省农垦总局资助(HNKXV-08-01)

摘　　要：猪应激综合征(ProcineStressSyndrome,PSS)是指猪在应激因子的作用下发生恶性高热综合症(MalignantHyper thermiaSyndrome, MHS)。试验随机选取18头纯种野猪提取基因组DNA进行聚合酶链式反应,扩增得到RYR1 基因特异片段,经过HhaⅠ酶切阳性鉴定可断定这18头猪的RYR1 基因都没有发生突变,因此不存在猪应激综合征问题。boars were selected from practicing of College of Animal Science and Technology of August First Reclamination of Heilongjiang.Porcine stress syndrome (PSS),also known as malignant hyperthermia (MH),causes pale soft exudative meat,stress death and sensitivity to halothane in pigs. PSS has been known to be a genetic disease controlled by a recessive gene(n)at the autosomal locus(Hal).Among three genotypes (N/N,N/n and n/n)only recessive homozygous(n/n)can be detected by the halothane Challenge Test,which fails to distinguish heterozyous carriers(N/n)from homozygous(N/N).Our study found that preparation for polymerase chain reaction (PCR) amplymerase indentified quickly on the all of farm in our country by the halothane test.

关 键 词：野猪 聚合酶链反应 氟烷基因 应激综合征 PCR 

分 类 号：S828.2[农业科学—畜牧学]