重组人生长激素对中性粒细胞凋亡和存活的影响  

Effect of recombinant human growth hormone on apoptosis and survival of polymorphonuclear neutrophils

作　　者：吴新海[1] 屠伟峰[2] 郄文斌[2] 杨传红[2] 杨太成[2] 曾因明[1] 

机构地区：[1]徐州医学院江苏省麻醉医学重点实验室,221002 [2]广州军区广州总医院全军临床麻醉中心,510010

出　　处：《实用医学杂志》2005年第8期773-775,共3页The Journal of Practical Medicine

基　　金：本课题为广东省医学科研基金资助项目(编号:A2001518)

摘　　要：目的:研究重组人生长激素(rhG)对有(或)无H LPS刺激的离体培养中性粒细胞(PM N s)凋亡和存活的影响。方法:采健康人外周静脉血,血浆-Percoll不连续密度梯度离心法分离出PM N s,分为4组:对照组,rhG H(100ng/m L)组,LPS100ng/m L组,LPS100ng/m L刺激1h后加rhG H100ng/m L组,离体培养3h和24h上流式细胞仪检测PM N s的凋亡(A nnexin V法)。结果:无LPS刺激状态下,rhG H可引起PM N s早期凋亡率减少、存活率增加,但不影响晚期凋亡/死亡率;有LPS刺激状态下,rhG H不影响PM N s早期凋亡率、晚期凋亡/死亡率和存活率,而且可能对LPS对PM N s刺激有一定阻抑作用。结论:无LPS刺激状态下,rhG H可延迟PM N s凋亡、增强PM N s活力;与LPS共同孵育时,rhG H不影响PM N s凋亡和活力,且对LPS对PM N s的刺激有一定阻抑的作用。Objective To investigate the effects of recombinant human growth hormone(rhGH) on the apoptosis and the survival of polymorphonuclear neutrophils(PMNs) stimulated or unstimulated by lipopolysaccharide(LPS). Methods The peripheral venous blood was collected from healthy volunteers. Isolation of PMNs were performed by using the discontinuous plasma Percoll gradient technique. PMNs were divided into four groups:control,rhGH(100 ng/mL),LPS(100 ng/mL) and LPS(100 ng/mL)+rhGH(100 ng/mL). PMNs were cultured in enriched RPMI 1640 media at 2×106/mL for 3 and 24 h. PMNs apoptosis was confirmed by flow cytometry(Annexin V FITC protein binding assay). Results rhGH can delay the early apoptosis of PMNs and prolong its survival rate without LPS sitimulation. rhGH have showed no effects on the apoptosis and the survival of PMNs stimulated by LPS but some inhibitory effect of rhGH on PMNs stimulated by LPS. Conclusion rhGH could delay the apoptosis of PMNs and enhance the activity of PMNs, but not only lose the effects of delaying PMNsapoptosis and enhancing the activity of PMNs but also inhibit some stimulated effects of LPS on PMNs.

关 键 词：重组人生长激素 中性粒细胞凋亡 PMNS 密度梯度离心法 流式细胞仪检测 RHGH 离体培养 LPS 外周静脉血 凋亡率 阻抑作用 存活率 健康人 对照组 mL 24h 状态 死亡率 早期 晚期 活力 

分 类 号：R96[医药卫生—药理学]