OsNHX1基因转化84K杨的研究  

Transformation of Poplar 84K with OsNHX1 Gene

作　　者：王树耀[1] 田宗城[1] 王云[1] 周芳[1] 

机构地区：[1]湖南文理学院生命科学系,湖南常德415000

出　　处：《湖南文理学院学报（自然科学版）》2005年第1期60-63,共4页Journal of Hunan University of Arts and Science(Science and Technology)

摘　　要：采用农杆菌介导的方法开展了水稻Na+ /H+ 逆向转运蛋白基因OsNHX1转化84K杨的研究.建立了84K杨的叶外植体高频再生系统,经诱导不定芽及诱导生根阶段卡那霉素连续筛选,获得了大量卡那霉素抗性转化植株.PCR检测和Southern杂交检测表明,OsNHX1基因已成功整合到84K杨基因组.耐盐实验表明,转基因植株能在2 0 0mmolNaCl条件下正常生长.OsNHX1 gene (Na +/H + antiporter gene of Oryza sativa)was introduced into poplar 84K with Agrobacterium tumefaciens-mediated transformation.High frequency regeneration system of leaf explant of poplar 84K has been established.Through successive selection in the period of shoot and root induction under high level kanamycin pressure, many resistant regenerated plants were obtained, PCR assay and Southern blot analysis showed that OsNHX1 gene was integrated successfully into the genome of poplar 84K,and salt tolerance test showed that transgenic plants grew normally at 200mmol NaCl.

关 键 词：84K杨 基因转化 高频再生系统 卡那霉素抗性 农杆菌介导 PCR检测 转基因植株 叶外植体 蛋白基因 逆向转运 Na^+ 诱导生根 杂交检测 转化植株 NaCl H^+ 不定芽 基因组 水稻 

分 类 号：S792.11[农业科学—林木遗传育种] S511[农业科学—林学]