内皮素对血管平滑肌细胞中—氧化氮合成酶基因表达的影响  

Effect of Endothelin on Expression of Nitric Oxide Synthetase of Vascular Smooth Muscle Cell Gene

作　　者：周洪[1] 张晨晖[1] 李倩虹[1] 牛大地[1] 张新波[1] 温进坤[1] 周爱儒[1] 

机构地区：[1]北京医科大学心血管基础研究所分子生物学研究室,北京100083

出　　处：《中国高血压杂志》1994年第2期75-77,共3页

摘　　要：应用反转录－聚合酶链式反应（reversetranscription－polymerasechainreaction，RT-PCR）方法，测定出一氧化氮合成酶（nitricoxidesynthetase，NOS）mRNA不仅在心血管系统细胞中广泛存在，并且发现内皮素可以促进血管平滑肌细胞中NOSmRNA的表达（＋34．8％），增高血管平滑肌细胞中NOS酶活性（＋74％）。结果提示，由内皮素引起的血管平滑肌细胞中NOS基因表达和NOS活性的增加，可能是高血压中的一个重要的负反馈因素。With reverse transcripti0n-polymerase chain reaction (RT-PCR),it has been found that NOS mRN;\ is widely distributed in cells of car<liovascular system such as t'ardiomyocytes, endothelia1 ce]ls, vascular smooth musclc cel1s (VSMC ). When ET (1O-' mol/L ) was added to cu1tured VSMC, it significant1y increased the NOS mRNA leve1 in VSMC (+ 34. 8% ). By measuring the enzyme activity of NOS in cultured VSMC,it was f0und that when VSMC was treated with ET (2 X 10wv mol/L) l NOS activati0n was significantly increased (79% ) compared to the contr0l. These data show that the increased expression of NOS gene inVSMC induced by ET may be an important regulat0ry factor for hypertension.

关 键 词：内皮素 一氧化氮 合成酶 高血压 

分 类 号：R544.102[医药卫生—心血管疾病]