对《中国药典》2000年版Ⅰ部白芍中芍药苷含量测定方法的探讨  

Discussion about Determination of Paeoniflorin in Radix Paeoniae Alba in the Pharmacopeia of the People's Republic of China (2000 Ed) Volume I

作　　者：李冬雪[1] 杜守颖[1] 贾海英[1] 吴清[1] 郑海云[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《时珍国医国药》2005年第4期311-312,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的:改进白芍中芍药苷的含量测定方法。方法:比较了《中国药典》方法和改进方法。采用70%乙醇为提取溶剂, 流动相为甲醇-水(30:70),检测波长为230 nm。结果:此方法线性关系良好(r=0.999 5),平均加样回收率为101.518%, RSD为1.682%。结论:改进的方法简便,准确,可减少有机溶剂对人的毒害和对环境的污染,为实践中芍药苷的含量测定方法提供参考依据。Objective: The purpose is to establish a method for determination of paeoniflorin in Radix Paeoniae Alba. Methods:Compare the new method and the routine one. Extracting with 70% EtOH,a mixture of methanol-water(30 : 70) as the mobile phase,detection wavelength is 230 nm. Results: The linearity of this method was good(r = 0. 999 5). The average recovery of the added sample was 101. 518% and RSD was 1. 682%. Conclusion:The new method is simple, accurate and it can protect the environment from pollution. It can be used for the determination of Paeoniflorin of Paeonia lactiflora Pall.

关 键 词：高效液相色谱 白芍 芍药苷 含量测定 

分 类 号：R284.2[医药卫生—中药学]