青蒿琥酯对高温内毒素复合因素下巨噬细胞吞噬功能的影响  

Experimental study of artesunate on phagocytosis of macrophage with compound influence of hyperthermia and lipopolysaccharide

作　　者：谭庆[1] 罗炳德[1] 张培[1] 周爱军[1] 杨光[1] 李文[1] 

机构地区：[1]第一军医大学高温医学研究室,广东广州510515

出　　处：《中国工业医学杂志》2005年第2期98-100,共3页Chinese Journal of Industrial Medicine

摘　　要：目的　研究青蒿琥酯(AR)对高温内毒素(LPS)复合因素下巨噬细胞吞噬功能的影响。方法　采用体外培养的巨噬细胞系RAW2 64 7细胞,分成空白对照组、AR组、LPS组及AR +LPS组,分别于3 7℃和40℃,含95 %O2 、5 %CO2 条件下培养1、2、3、4h后,台盼蓝染色计数细胞存活数,吞噬中性红实验检测巨噬细胞的吞噬功能。结果　经不同条件处理3h后各组细胞存活数无差异,均大于95 %;高温内毒素复合因素使巨噬细胞的吞噬功能明显降低,青蒿琥酯亦使巨噬细胞的吞噬功能下降,但青蒿琥酯加内毒素组的吞噬功能明显强于单纯内毒素组。结论　青蒿琥酯对高温内毒素复合因素下的巨噬细胞的吞噬功能有增强作用。Objective To investigate the effects of artesunate(AR)on phagocytosis of macrophage with compound influence of hyperthermia and lipopolysaccharide(LPS).Method A macrophage cell line RAW264.7 was employed in an in vitro experiment and cells were divided into blank control group,AR group,LPS group and AR+LPS group.Cells were incubated for 1,2,3 and 4 hours at 37 ℃ or 40 ℃ with 5%CO_2,respectively,and stained with trypan blue for counting of survival cells,and neutral red absorption test was used to observe phagocytosis function of macrophages.Result No significant difference in survival rate(more than 95%)of macrophages was observed in all the groups 3 h after different treatments.Phagocytosis of macrophages decreased significantly by AR and under compound influence of hyperthermia and LPS,but phagocytosis in cells treated with both AR and LPS was significantly stronger than that in those treated with LPS only.Conclusion AR could evidently enhance phagocytosis of macrophages under compound influence of hyperthermia and LPS.

关 键 词：高温 内毒素 巨噬细胞 吞噬功能 青蒿琥酯 

分 类 号：R135.3[医药卫生—劳动卫生]