TZD对糖尿病肾病中细胞凋亡及相关基因bcl-2/bax的影响  

The effect of TZD on apoptosis and ITS Bax/bcl-2 in the kidney tissue of diabetic rats

作　　者：郭延军[1] 李云[1] 李海华[1] 杨宝峰[1] 刘海燕[1] 李秀梅[1] 邓华聪[2] 

机构地区：[1]大庆市第一医院,黑龙江大庆163001 [2]重庆医科大学附属第一医院

出　　处：《中国实验诊断学》2005年第2期249-252,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的　探讨细胞凋亡在糖尿病肾病中的作用以及吡格列酮(TZD)对糖尿病肾病中细胞凋亡的影响。方法　建立链脲佐菌素(STZ)诱导的糖尿病动物模型,随机分为正常对照组(NC)、正常对照治疗组(NCT)、糖尿病组(DM)、糖尿病治疗组(DMT)。NCT组和DMT组均给予TZD(3mg/kg d)灌胃治疗。采用免疫组织化学方法(S P法)检测大鼠肾组织bcl- 2及BAX蛋白表达,原位末端标记(TUNEL)法检测大鼠肾组织细胞凋亡指数,电镜下观察大鼠肾组织超微结构的改变。结果　DM大鼠肾细胞凋亡指数较其他3组肾组织高(P <0 .0 5 ) ,各组大鼠肾组织中bcl- 2以及BAX均有不同程度的蛋白表达,DM大鼠肾组织BAX、bcl -2蛋白表达高于其他3组(P <0 . 0 5 )。电镜见DM大鼠肾组织有较多的系膜细胞核内染色质趋边浓缩,聚集等细胞凋亡的形态学改变。结论　bcl- 2和BAX蛋白均参与糖尿病肾病组织中细胞凋亡的调控,并在糖尿病肾病的发生发展中发挥重要作用。TZD通过影响BAX /bcl- 2蛋白表达抑制细胞凋亡,是其对糖尿病大鼠肾脏具有保护作用的机制之一。Objective To explore the changes of apoptosis in diabetic nephropathy and the effect of TZD on apoptosis in the kidney tissue of diabetic rats. Methods The animal model was induced by STZ. Wistar rats were randomly divided into following groups:normal control group(NC); normal control group with TZD(NCT); diabetic mellitus group(DM); DM group with TZD(DMT). NCT and DMT were treated with TZD(3 mg/kg.d) by gavage. The bcl-2/bax proteins of rats ronal tissue were detected by immunohistochemical methods(s-p). The apoptosis index of renal tissue was detected by the terminal deoxynucleotidyl transferase mediated nick end labeling, the ultrastructure of renal tissue was observed by electromicroscope in rats. Results Apoptosis index of diabetic group was significantly increased when compared with that of other three group( P <0.05). The expression of bcl-2 protein and BAX protein in kidney tissue of each group were presented in various degree. Pyknotic nuclei of mesangial cell, Which is the typical morphological features of apoptosis, were increased in kidney tissue of rats under eletromicroscope. Conclusion Both bcl-2 and BAX proteins take part in the occurrence and progression of diabetic nephropathy. TZD can inhibit apoptosis of kidneys on diabetic rats through regulating the balance of expression of Bax/bcl-2. It is one of the mechanism of TZD protective effect on diabetic kidney.

关 键 词：糖尿病肾病 细胞凋亡 BCL-2/BAX 

分 类 号：R587.2[医药卫生—内分泌]