抑肽酶在转基因烟草中的表达  

Expression of Aprotinin Gene in Tobacco

作　　者：耿学军[1] 常伟勤[2] 范伟全[1] 杨莉莉[1] 贺巾超[1] 马杰[1] 颜炜群[1] 

机构地区：[1]吉林大学再生医学科学研究所,吉林长春130021 [2]吉林大学第二附属医院

出　　处：《中国实验诊断学》2005年第2期282-284,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的　在烟草中高效表达抑肽酶(bpti)。方法　克隆抑肽酶基因,以烟草叶片为外植体,通过根癌农杆菌将其导入烟草,经卡那霉素抗性筛选和PCR扩增检测基因的整合;抽提转染植株叶片中的抑肽酶,进行活性测定。结果　抗性植株整合并高效表达了抑肽酶。结论　构建的含抑肽酶基因的植物表达载体pBI1 2 1 +bpti具有正确的阅读框,并能在异源植物中表达。Objective To clone and express recombinant aprotinin in tobacco. Methods Aprotinin gene was synthesized according to the bias in plant codon usage. Leaf segments of tobacco were infected by Agrobacterium tumefaciens EHA105 with plasmid pBI121bpti. The activity of product was measured. Results 37 kanamycin resistant plants integrating the aprotinin gene(pBI121bpti) was obtained, and the recombinant aprotinin was induced. Conclusion The aprotinin gene could be integrated into the genome of tobacco correctly, and expressed in heterogenous plants.

关 键 词：抑肽酶 表达载体 烟草 

分 类 号：S572[农业科学—烟草工业]