Analysis of Toxin β-N-Oxalyl-α, β-diaminopropionic Acid (β-ODAP),its Isomer α-ODAP and Other Free Amino Acids in Lathyrus sativus  

作　　者：ZeYiYAN ChengJinJIAO FenMinLI YongMinLIANG ZhiXiaoLI 

机构地区：[1]StateKeyLabor_atoryofAppliedOrganicChemistry,LanzhouUniversity,Lanzhou730000 [2]Schooloflifescience,LanzhouUniversity,Lanzhou730000

出　　处：《Chinese Chemical Letters》2005年第5期627-630,共4页中国化学快报（英文版）

基　　金：This work was supported by NKBRSF Project(G200001 8603);the National Natural Science Foundation of China(No.30270965).

摘　　要：A method has been presented for the simultaneous analysis of neurotoxin β-N-oxalyl-α, β-diaminopropionic acid (β-ODAP) and its non- or low-toxic isomer α-ODAP as well as other amino acids in Lathyrus sativus extracts after derivatization with para-nitrobenzyloxycarbonyl chloride (PNZ-Cl) by reversed-phase (C18 column) high performance liquid chromatography (RP-HPLC). Detection limits of isomers were 0.08 μg/mL for both β-, α-ODAP, for homoarginine 0.09 μg/ mL at a signal-to-noise ratio of 3:1.

关 键 词：HPLC β-N-oxalyl-L-α β-diaminopropanoic acid α-ODAP HOMOARGININE PNZ-Cl. 

分 类 号：Q949.751.9[生物学—植物学]