Capillary Zone Electrophoresis Investigation of Interactions between Granulocyte-colony Stimulating Factor and Dextran Sulfate/Carrageenan Oligosaccharide  

作　　者：AiYeLIANG YuGuangDU KeYiWANG BingChengLIN 

机构地区：[1]DalianInstituteofChemicalPhysics,ChineseAcademyofSciences,Dalian116023 [2]InstituteofBiochemistryandCellBiology,ShanghaiAcademyofLifeScience,ChineseAcademyofSciences,Shanghai200031

出　　处：《Chinese Chemical Letters》2005年第5期647-650,共4页中国化学快报（英文版）

基　　金：The authors would like to acknowledge the support from the National Natural Science Foundation of China(Project number 20299035,20035010,20275039);Pilot of Knowledge Innovation Program of the Chinese Academy of Science(KSCX 2-3-02-02)on the above work.

摘　　要：The interactions between granulocyte-colony stimulating factor (G-CSF) and dextran sulfate / κ-carrageenan oligosaccharide were studied by capillary zone electrophoresis. Dextran sulfate could strongly interact with G-CSF and the complex was detected. The binding constant and stoichiometry were determined to be 1.2×106 (mol/L)-1 and 3:1, respectively. However, the interaction between κ-carrageenan oligosaccharide and G-CSF was not found.

关 键 词：Capillary zone electrophoresis granulocyte-colony stimulating factor dextran sulfate κ-carrageenan oligosaccharide INTERACTION 

分 类 号：Q592.1[生物学—生物化学] O636.1[理学—高分子化学]