牛血清白蛋白乳酸-羟乙酸共聚物微球的制备  

Preparation of BSA Poly(Lactide-co-glycolide acid) Microparticles

作　　者：陈善[1] 李萍 丁建新 陶胜源[2] 窦媛媛 王希良 王洪权 

机构地区：[1]中国人民解放军军事医学科学院微生物流行病研究所,北京100071 [2]中国人民解放军总后勤部卫生部药品仪器检验所,北京100071

出　　处：《解放军药学学报》2005年第2期96-100,共5页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的　以牛血清白蛋白为模型蛋白,以乳酸羟乙酸共聚物(PLGA)为包裹材料,探索粒径小于10 μm的微球的制备方法并优化工艺。方法　采用复乳溶剂挥发法制备蛋白质微球,以BCA法及微量BCA法测定微球的蛋白含量及蛋白从微球的释放。考察BSA浓度、内外相体积比、PLGA浓度、超声功率、匀浆转速、PVA浓度、PVA体积、PLGA分子量等因素对微球包封率、粒径、载药量及突释量的影响。结果　通过控制不同的因素,可以得到较高的载药量及包封率、粒径在5 μm左右的微球。结论　采用复乳溶剂挥发法通过控制不同的因素,可得到粒径5 μm左右不同载药量及突释量的具有较高包封率的微球。Aim To study the method of preparation of BSA micros ph ere with diameter within 10μm and optimize the preparation techniques and presc ription composition.Methods Microparticles were pnepared using a(wate r-in-oil)-in-water emulsion solvent evaporation techique.The concentra tion of BSA and the releasing of BSA were determined by BCA and micro BCA method .Study how the c oncentration of BSA,volume ratio of internal and outer phase,the concentration o f PLGA,the power of sonication,the homogenization speed,the concentration of PVA ,the volume of PVA and the molecular weight of PLGA to affect the particle size, entrapment efficiency and BSA entrapment of microparticles.Results Smooth spherical microparticles 3～5μm in diameter containing relatively hig h concentration of BSA could be produced by controlling different process parame ter.Conclusion The desirable microparticles 3～5μm in diameter containing relatively high concentration of BSA can be produced by controlled d ifferent process.

关 键 词：牛血清白蛋白 乳酸-羟乙酸共聚物(PLGA) 溶剂挥发法 BCA法 蛋白质微球 包封率 载药量 包裹材料 优化工艺 制备方法 蛋白含量 超声功率 PVA 粒径 浓度 BSA 相体积 等因素 分子量 复乳 突释 控制 

分 类 号：R979.1[医药卫生—药品] R944.1[医药卫生—药学]