哌拉西林与加替沙星联用对铜绿假单胞菌的联合药敏研究  

The Study on the Antibacterial Activity of Piperacillin Combine with Gatifloxacin Against Pseudomonas Aeruginosa

作　　者：任浩洋[1] 周筱青[1] 裴斐[1] 王睿[1] 柴栋[1] 方翼[1] 

机构地区：[1]中国人民解放军总医院药材处,北京100853

出　　处：《解放军药学学报》2005年第2期121-123,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的　评价哌拉西林与加替沙星联合用药,对30株临床分离的铜绿假单胞菌的体外抗菌效应。方法　采用棋盘法设计,微量肉汤稀释法测定。测定不同浓度组合的抗菌药物对30株临床分离的铜绿假单胞菌的最低抑菌浓度,并计算FIC指数判定联合效应。FIC≤0 .5为协同作用,0 .5 <FIC≤1为相加作用,1<FIC≤2为无关作用,FIC >2为拮抗作用。结果　哌拉西林与加替沙星联用后,其MIC50 显著降低。FIC指数分布:FIC≤0 .5占4 0 % ;0 .5 <FIC≤1占5 0 % ;1<FIC≤2占10 % ;FIC >2为0。结论　结果表明两药联用,对铜绿假单胞菌表现为协同作用和相加作用,并以协同作用为主,无关作用较少,更无拮抗作用。Aim To evaluate the antibiotic effects of piperacill in combined with gatifloxacin against 30 strains of Pseudomonas aeruginosa. Methods The protocol was designed by checkerboard method and the MICs of piperacillin or/and combined with other 6 antibiotics against the 30 strains of Pseudomonas aeruginosa were determined by broth dilution method,the FIC index was calculated according to MIC results.Results The perc entage of the FIC indexes less than 0.5,from 0.5 to 1,from 1 to 2,more than 2 we re 40%,50%,10%,0% respectively.Conclusion The results suggested t hat synergism and additivity of piperacillin combined with gatifloxacin respecti vely against 30 strains of Pseudomonas aeruginosa were main action,there wer e little autonomy and no antagonism.

关 键 词：铜绿假单胞菌 加替沙星 哌拉西林 药敏研究 联用 微量肉汤稀释法 体外抗菌效应 最低抑菌浓度 协同作用 临床分离 相加作用 拮抗作用 FIC指数 MIC50 联合用药 抗菌药物 不同浓度 联合效应 指数分布 棋盘法 测定 

分 类 号：R378.991[医药卫生—病原生物学] R978.1[医药卫生—基础医学]