地花菌提取物Albaconol的抗肿瘤活性研究  

Study on antitumor activity of albaconol extracted from Albatrellus confluens

作　　者：刘明华[1] 肖顺汉[1] 秦大莲[1] 章卓[1] 卿晨[2] 张雁丽[2] 

机构地区：[1]泸州医学院药理学教研室 [2]昆明医学院省天然药物药理重点实验室,泸州646000

出　　处：《中药药理与临床》2005年第1期14-16,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金地区重点项目 (No 399690 0 5);云南省自然科学基金重点项目 (No 2 0 0 0C0 0 0 1P)

摘　　要：目的:研究地花菌提取物albaconol的抗肿瘤活性。方法:以人白血病K 5 62、人乳腺癌Bcap 3 7、人胃腺癌BGC 82 3和人非小细胞肺癌A5 49为模型,采用MTT法测试albaconol对肿瘤细胞增殖的影响;以小鼠肉瘤S180和小鼠肝癌H2 2为移植性肿瘤模型,检测albaconol静脉给药对肿瘤生长的影响。结果:Albaconol对K5 62、Bcap 3 7、BGC 82 3、A5 49的IC50 分别为2 .42±1.77、1.88±1.41、1.0 4±0 .64、1.18±1.10 μg/ml ;Albaconol 0 .87、1.73、3 .46mg/kg剂量组对S180的抑瘤率分别为2 8.2 %、43 .2 %、47.4% ,对H2 2的抑瘤率分别为15 .6%、2 2 .4%、3 7.8%。结论:Albaconol具有较强的体内、外抗肿瘤活性。Objective: To study the antitumor activity of albaconol extracted from Albatrellus confluens in vitro and in vivo. Methods: The cytotoxicity of albaconol on human leukemia K-562, breast carcinoma Bcap-37, gastric adenocarcinoma BGC-823 and lung adenocarcinoma A549 cell lines were determined by MTT assay and the results were expressed as IC 50 values. The inhibitory effect of albaconol on tumor growth was observed by the models of implanted sarcoma 180 (S180) and hepatoma 22 (H22) in mice and the results were expressed as inhibitory rate of tumor growth. Results: The IC 50 values of albaconol on K562、Bcap-37、BGC-823 and A549 cell lines were 2.42±1.77、1.88±1.41、1.04±0.64 and 1.18±1.10μg/ml, respectively. The inhibitory rates of tumor growth at doses of 0.87、1.74、3.48 mg/kg of albaconol on S180 and H22 were 28.2%、43.2%、47.4% and 15.6%、22.4%、37.8% respectively. Conclusion: Albaconol exhibited significant antitumor activity in vitro and in vivo.

关 键 词：提取物 活性研究 BCAP-37 BGC-823 白血病K562 人非小细胞肺癌 抗肿瘤活性 肿瘤细胞增殖 肉瘤S180 A549 肝癌H22 人乳腺癌 人胃腺癌 MTT法 肿瘤模型 肿瘤生长 IC50 抑瘤率 移植性 小鼠 

分 类 号：R285[医药卫生—中药学]