小鼠血浆中芍药苷提取分离方法筛选  

Study on the method of pretreatment of plasma containing paeoniflorin for detection by HPLC

作　　者：刘荣敏[1] 程佳[1] 杨祖贻[1] 裴瑾[2] 万德光[2] 胡荣[2] 

机构地区：[1]四川省肿瘤研究所,成都610041 [2]成都中医药大学药学院,成都610075

出　　处：《中药药理与临床》2005年第1期63-64,F003,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家中医药管理局科研基金 (0 2 0 3ZP5 0 ) ;四川省中医管理局科研基金 (2 0 0 2 17)

摘　　要：目的:通过对三种体外小鼠血浆中微量芍药苷提取分离方法的比较,从中筛选出一种最好的方法,将其用于体内生物样品中微量芍药苷的提取分离。方法:在小鼠血浆中外加一定量的芍药苷,作用一定时间后,分别用甲醇、乙醇、加热法提取血浆中微量芍药苷,进行HPLC检测。结果:甲醇、乙醇、加热法等三种方法结果以乙醇法回收率最高。结论:血浆中微量芍药苷提取分离以乙醇法适用于小鼠实验。Objective: To develop a method of pretreatment of plasma for detection paeoniflorin in plasma in mice. Methods: Content of peaoniflorin in plasma, which had been treated with methanol, ethanol or boiling water bath respectively, was determined directly by HPLC. Results: When eluted by mobile phase of CH 3OH-H 2O (38:62),and stationary phase of C18,column(250mm×4.6mm,7μm),the minimal detectable was 1.49 ng/μl, the average recovery was 95.52%. Conclusion: In comparing with there samples preparation, the method of ethanol treated plasma is simple, rapid, sensitive, accurate ,specific, reproducible and no toxic.

关 键 词：鼠血浆 方法筛选 中芍药苷 提取分离方法 HPLC检测 生物样品 小鼠实验 加热法 乙醇法 微量 回收率 甲醇 

分 类 号：R285.5[医药卫生—中药学]