甲壳胺及N—乙酰葡糖胺抑制人成纤维细胞增殖的研究  

A STUDY ON CHITOSAN INHIBITING FIBROBLAST PROLIFERATION IN VITRO

作　　者：黄亦武[1] 陆放[1] 

机构地区：[1]上海第二医科大学附属新华医院,上海200092

出　　处：《中国海洋药物》1994年第2期36-37,共2页Chinese Journal of Marine Drugs

摘　　要：本文报道了甲壳胺及N—乙酰葡糖胺对体外培养的人成纤细胞的抑制作用以及甲壳胺在动物体内的生物降解。研究结果表明,N—乙酰葡糖胺和甲壳胺的抑制作用分别为ID_(50)=16.35mg/L,和ID_(50)=69.59mg/L;经过25d和18d兔体内埋植观察,甲壳胺分子量下降分别约为17％和6％。这一现象提示,甲壳胺在长效抑制人纤维增殖方面有潜在的应用价值。We use chitosan and n -acetyl -d -glucosamine to inhibit fibroblast proliferation which was cultured in vitro. The result shows that n - acetyl - d - glucosamine: ID50=16. 35mg/L, chitosan: ID50=69. 59mg/L. We also plant the chitosan membrane to the rabbit body to test its bio-degradation. The result shows that the molecular weight of chitosan drops 17% and 6% after 25 days and 18 days, respectively. On the basis of these results, we have obtained a conclusion that we can probably use chitosan to continuously release n-acetyl -d-glucosamine which can inhibit fibroblast proliferation effectively.

关 键 词：甲壳胺 N-乙酰葡糖胺 生物降解 人成纤维细胞 

分 类 号：R282.770.5[医药卫生—中药学]