三种免疫学方法检测班氏丝虫微血蚴血症者抗体的比较  

COMPARATIVE STUDY ON THREE SEROLOGIC TESTS FOR DETECTING ANTIBODIES IN PERSONS WITH BANCROFTIAN MICROFILAREMIA

作　　者：孙德建[1] 袁以真[1] 施恒华[1] 罗曼珍[1] 陈韶红 史宗俊[1] 

机构地区：[1]中国预防医学科学院寄生虫病研究所

出　　处：《中国寄生虫学与寄生虫病杂志》1994年第3期185-187,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：本文报告应用间接荧光抗体试验（ＩＦＡＴ）、酶联免疫吸附试验（ＥＬＩＳＡ）和斑点－酶联免疫吸附试验（ｄｏｔ－ＥＬＩＳＡ）对１０２份班氏微丝蚴血症者血清同时进行对比检测的结果。ＩＦＡＴ、ＥＬＩＳＡ和ｄｏｔ－ＥＬＩＳＡ的抗体阳性率分别为８９．２％（９１／１０２）、８４．３％（８６／１０２）和８９．２％（９１／１０２）。３种方法检测非流行区正常人血清的阳性率分别为０（０／３０）、１．８％（１／５５）和０（０／５４）。检测的抗体水平与血中微丝蚴密度的高低无一定的相关关系。结果表明３种方法检测班氏丝虫微丝蚴血症者抗体的阳性率间无显著性差异，在丝虫病防治后期血清学监测工作中可选择应用。联合应用２或３种方法对提高检测的阳性率有互补作用。A comparative study was conducted for detecting antibody levels of bancroftian microfilaremia cases by using three serologic tests,IFAT,ELISA and dot ELISA.A total of 102 serum specimens were collected from bancroftian microfilaremia cases.The positive rate was 89.2%(91/102) with IFAT(serum dilution≥1∶20),84.3%(86/102)with ELISA(OD value≥0.440) and 89.2%(91/102) with dot ELISA(serum dilution≥1∶80),respectively ( P >0.05);while in healthy individuals from non endemic areas the false positive rate was 0(0/30),1.8(1/55) and 0(0/54),respectively.The results indicated that there were no statistically significant differences among the three serologic tests. There was no correlation found in this study between microfilaria density and the antibody level.It was also proved that the detection rate of the combined use of either 2 or 3 tests was higher than each of the three tests;the positive rates being 94.1% 98.0%.

关 键 词：丝虫病 血清学 ELISA IFAT 

分 类 号：R532.150.4[医药卫生—内科学]