鼻息肉中嗜酸性粒细胞浸润和活化与白细胞介素5表达  

The correlation between infiltration and activation of eosinophils and IL - 5 ex pression in nasal polyps

作　　者：张罗[1] 韩德民[1] 周兵[2] 张勇[3] 

机构地区：[1]北京市耳鼻咽喉科研究所,北京100005 [2]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,北京100730 [3]首都医科大学附属北京同仁医院病理科,北京100730

出　　处：《中国耳鼻咽喉头颈外科》2005年第3期171-174,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：北京市科委新星计划(2003–B-15);北京市卫生局归国人员科研基金;北京市委优秀人才培养专项经费

摘　　要：目的通过观察鼻息肉中嗜酸性粒细胞的浸润和活化状态,以及白细胞介素5(interleukin-5,IL-5)的表达水平,揭示二者的关系,探索鼻息肉中嗜酸性粒细胞浸润增多调节的机制。方法对30例鼻息肉组织,分别采用chromotrope2R法标记鼻息肉组织中的嗜酸性粒细胞,采用免疫组织化学ABC法,观察嗜酸性粒细胞的活化状态和IL-5的表达水平,然后进行相关性分析。结果①chromotrope 2R染色可特异性地将嗜酸粒细胞的胞浆染成粉红色,活化嗜酸性粒细胞(EG2阳性细胞)和IL-5阳性细胞胞浆中可见棕褐色颗粒。30例鼻息肉中嗜酸性粒细胞的密度为50.7±16.1/0.25mm2,活化嗜酸性粒细胞的密度为20.7±14.3/0.25mm2,IL-5阳性细胞密度为15.9±5.7/0.25mm2,上述三项指标在变应性患者和非变应性患者比较未见显著性差异(P>0.05);②鼻息肉中IL-5阳性细胞密度与chromotrope2R阳性细胞密度密切相关(y=14.723+2.012x,r=0.642,P<0.01),与EG2阳性细胞密度也存在相关关系(y=11.817+1.092x,r=0.602,P<0.05)。结论嗜酸性粒细胞是鼻息肉组织中IL-5的来源之一,IL-5的表达水平是鼻息肉中嗜酸性粒细胞的浸润和活化的调节因素之一。OBJECTIVE To assess the correlation between the infiltration and ac tivation of eosinophils and IL - 5 expression in nasal polyps. METHODS Nasal p olyps from thirty patients were investigated. The samples were stained with HE, chromotrope 2R histochemical and ABC immunohistochemical methods. RESULTS There was no difference in chromotrope 2R positive cells, EG2 positive cells and IL - 5 positive cells between allergic and nonallergic patients. The density of IL - 5 positive cells correlated with both the density of chromotrope 2R cells (y = 14.723 + 2.012 x,r = 0.642,P < 0.01) and the density of EG2 cells (y = 11.817 + 1.092 x,r = 0.602,P < 0.05). CONCLUSION The correlation both between IL - 5 exp ression and the infiltration and activation of eosinophils indicated the roles o f IL - 5 in the infiltration and activation of eosinophils in nasal polyps.

关 键 词：嗜酸性粒细胞浸润 白细胞介素5 免疫组织化学ABC法 鼻息肉组织 IL-5 细胞密度 活化状态 相关性分析 嗜酸粒细胞 显著性差异 细胞胞浆 阳性细胞 相关关系 调节因素 变应性 水平 粉红色 特异性 EG2 患者 

分 类 号：R765[医药卫生—耳鼻咽喉科]