螺旋藻的纯化  

Purification of Spirulina sp.

作　　者：甘旭华[1] 唐欣昀[1] 刘广金[1] 史成颖[1] 汪本凡[1] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036

出　　处：《微生物学通报》2005年第2期1-4,共4页Microbiology China

基　　金：安徽省科技厅自然基金资助(No.99042130);安徽省教育厅自然基金资助(No.97JL052)

摘　　要：观察了螺旋藻生长过程中藻丝和杂菌的生长规律,发现中性细菌和碱性细菌的数量始终是藻丝的105～106倍。采用常规的稀释平板法、毛细管法和挑单藻法均无法可靠地获得无菌纯藻。设计用低速离心法洗涤下沉性藻丝,用过滤法洗涤上浮性藻丝,对藻丝进行预处理洗去大量杂菌;对迁移性和非迁移性藻株分别采用夹层法和平板法纯化藻株,使得单根藻丝在平板上形成藻落,获得无菌纯藻。Growth patterns of trichome and contaminative bacteria in Spirulina sp. liquid culture were observed, and it was found that the number of neutral and alkalophilic bacteria was always 105～106 times of that of Spirulina sp. trichome. It would be very difficult to get real pure Spirulina sp. strain by classical methods of dilution plate, capillary and single trichome selecting methods. A great deal of contaminative bacteria was washed out by two pretreatment processes. Low speed centrifugation was designed to wash the strains which usually deposit at bottom, and filtration method was designed to treat the strains usually floating at surface. Sandwich plate and dilution plate were designed for the purification of the mobile strains and non-mobile strains, respectively. A lot of strains were purified by the above processes and pure single trichome formed pure colonies on plates.

关 键 词：螺旋藻 纯化技术 单藻落 

分 类 号：Q94-3[生物学—植物学]