对HCMVUL54 mRNA片段特异性切割的M1GS构建  

Construction of M1GS for Targeted Cleavage of HCMV UL54 mRNA Segments

作　　者：吕静竹[1] 李弘剑[1] 陈浩军[1] 李月琴[1] 周天鸿[1] 唐冬生[1] 张欣[1] 

机构地区：[1]暨南大学生命科学技术学院,广州510632

出　　处：《微生物学通报》2005年第2期83-86,共4页Microbiology China

基　　金：国家自然科学基金资助项目(No.30370776);广东省自然科学基金重大项目(No.36703);广东省自然科学基金(No.021162;000718)

摘　　要：人巨细胞病毒是一种DNA病毒,在人群中一般呈亚临床感染和潜伏感染。为研究病毒基因沉默工具和抗病毒制剂,以人巨细胞病毒UL54基因mRNA序列设计互补的外部引导序列,共价结合到大肠杆菌来源RNaseP催化核心M1RNA上,从而构建成M1GS-T6核酶。通过对DNA聚合酶UL54基因亚克隆片段转录产物体外切割研究,证实该核酶具备对UL54mRNA片段的特异切割能力。Human cytomegalovirus (HCMV) is a DNA virus and serious opportunistic pathogen for both newborn and immunocompromised individuals.To research technique for gene silence and antiviral agents, ribozyme M1GS-T6 was constructed from external guide sequences(EGSs)that consist of a sequence complementary to HCMV UL54 gene RNA and M1 RNA, the catalytic RNA subunit of RNase P from Escherichia coli. The results showed that M1GS can efficiently cleave the mRNA sequence encoding UL54 protein in vitro.

关 键 词：人巨细胞病毒 UL54基因 RNASE P 外部引导序列 

分 类 号：R346[医药卫生—基础医学]