基因表达常规分析方法概述  

Conventional Methods for the Analysis of Gene Expression: an Update Review

作　　者：赵子轶[1] 左天荣[1] 韦平[1] 

机构地区：[1]广西大学动物科技学院,广西南宁530005

出　　处：《生物技术通讯》2005年第2期204-206,共3页Letters in Biotechnology

基　　金：广西科学基金项目(桂科自0339003);国家自然科学基金项目(30460099)

摘　　要：在基因表达的分析中,RNA转录的稳态水平是检测细胞株和组织的基因表达活性的最方便的参数之一。本文概述了用于检测mRNA丰度的常规分析方法,即Northern印迹、RNase保护试验和实时定量PCR技术,特别是为对数量不多的基因进行表达情况的测定时提供了较为实用的方法。The conventional methods used in the identifications of the changes in the expression and living activity of genes in cell strain and tissue were reviewed here. A variety of methods have been developed for analyzing mRNA expression. Among these techniques, Northern blot, ribonuclease protection assay(RPA), real-time quantitative polymerase chain reaction(RQ-PCR), which are based on differentially screening and hybridization technology, have been applied most frequently.

关 键 词：基因表达 NORTHERN印迹 RNase保护试验 实时定量PCR技术 分析方法 

分 类 号：Q786[生物学—分子生物学]