声诺维(Sono Vue)增强超声辐照对HepG2细胞膜通透性的影响  

HepG2 membrane permeability effected by SonoVue enhanced ultrasound exposure

作　　者：岳殿超[1] 徐辉雄[1] 吕明德[2] 汤庆[1] 李宝金[2] 向邦德[2] 

机构地区：[1]中山大学附属第一医院医学影像部,广东广州510080 [2]中山大学附属第一医院肝胆外科,广东广州510080

出　　处：《中国医学影像技术》2005年第4期510-512,共3页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金(30300082; 30470467);广东省自然科学基金(04009360)。

摘　　要：目的　探讨超声辐照对人肝癌细胞株HepG2 细胞通透性的影响以及声学造影剂声诺维(SonoVue)的增强作用。方法　用连续多普勒超声辐照人肝癌细胞株HepG2,超声发射频率1.9 MHz,软组织热指数(TIS)为0.8,增益设定为80%,输出声强(ⅠSATA)为80.0 mW/cm2,辐照时间均持续60 s。照射时添加或不添加超声造影剂声诺维(SonoVue)。用流式细胞仪检测细胞荧光染色阳性的阳性率。结果　荧光染色阳性细胞的百分率:无超声辐照组或单纯添加SonoVue组分别为(3.06±1.57)%和(1.4±0.04)%,经超声辐照后为(23.72±14.4)%,添加SonoVue辐照后显著增加至(54.78±43.6)%(均P<0.05)。结论　超声辐照能改变HepG2细胞膜通透性,添加超声造影剂后能显著增强这种作用,有希望成为肿瘤分子生物学治疗的介导手段。Objective To explore the role of ultrasound radiation on the permeability of HepG2 cell with/without the intervention of ultrasound contrast SonoVue. Methods HepG2 cell suspension infused with Fluorescent dextran molecules (FD500) was exposed to CW Doppler ultrasound, 1.9 MHz, Tis 0.8, gain 80%,Ⅰ SATA 80.0 mW/cm 2, with/without SonoVue infused. Flow cytometry was used to detect the fluorescent rate. Results The uptake rate of fluorescent cells in control groups (pseudo-US exposure group and only with micro-bubble contrast agent group) were (3.06±1.57)% and ( 1.4 ±0.04)% respectively. Significant uptake increases were found in the US exposure group ([23.72±14.4]%) and US with micro-bubble contrast group ([54.78±43.6]%). Conclusion Diagnostic ultrasound destruction of contrast-agent microbubbles can enhance the permeability of HepG2 cell membrane, which contributes to the delivery of large molecules. It maybe become a promising biomolecular therapeutic method on oncology.

关 键 词：超声造影剂 微泡 空化效应 癌 肝细胞 膜通透性 

分 类 号：R445.1[医药卫生—影像医学与核医学] O426.4[医药卫生—诊断学]