慢性心房颤动对心房肌细胞内游离钙浓度的影响  

The effect of chronic atrial fibrillation on intracellular free calcium concentration of human atrial myocytes

作　　者：陈劲进[1] 肖颖彬[1] 刘健[1] 

机构地区：[1]第三军医大学新桥医院心血管外科,重庆400037

出　　处：《临床心血管病杂志》2005年第4期204-206,共3页Journal of Clinical Cardiology

基　　金：国家自然科学基金资助项目(调控Af心房电重构基因表达的L型钙通道信号通路研究No:30370583和心肌重塑Ca~(2+)转录耦联的信号转导机制No:30200108)

摘　　要：目的探讨慢性心房颤动(Af)对心房肌细胞内游离Ca2+的影响。方法用激光共聚焦显微镜技术,以Fluo4/AM作为钙指示剂,对急性分离的慢性风湿性心脏病伴慢性Af、窦性心律患者的心房肌细胞内游离Ca2+浓度进行测定,观察慢性风湿性心脏病伴慢性Af患者心房肌细胞是否存在钙超载。结果慢性风湿性心脏病伴慢性Af患者心房肌细胞内游离Ca2+浓度显著高于窦性心律患者[(276.38±38.12)∶(122.28±45.63)nmol/L,P<0.05]。结论慢性Af患者心房肌细胞内存在钙超载。Objective:To investigate the effect of chronic atrial fibrillation(Af) on intracellular free Ca^2+ concentration of atrial myocytes. Method: Intracellular free Ca^2+ concentration in acute isolated atrial myocytes of rheumatic heart disease patients with Af and with normal sinus rhythm was measured with laser scanning cofocal microscopy technique using the fluorecent Ca^2+ indicator Fluo-4/AM. Result:The intracellular Ca^2+ concentration of patients with Af was significantly higher than that of patients with normal sinus rhythm[ (276.38±38.12) vs (122.28±45.63)nmol/L, P<~0.05 ]. Conclusion: Chronic Af can lead to intracellular calcium overload in human atrial myocytes.

关 键 词：心房颤动 心房肌 钙超载 

分 类 号：R541.7[医药卫生—心血管疾病]