抗HTNV单抗1A8鼠/人嵌合Fab抗体植物表达载体的构建  

Construction of Plant Expression Vector Containing Mouse/Human Chimeric Fab Gene Fragment of mAb 1A8 Against HTNV

作　　者：罗雯[1] 白文涛[2] 吴兴安[2] 张九东[1] 

机构地区：[1]西安文理学院生命科学系,西安710065 [2]第四军医大学微生物学教研室,西安710032

出　　处：《科学技术与工程》2005年第9期552-555,共4页Science Technology and Engineering

基　　金：国家自然科学基金(30471626)西安市科技局社会与发展计划(200345)西安文理学院专项科研基金(KY2004024)资助

摘　　要：利用PCR方法,从含有1A8VLCκ基因或1A8Fd基因的重组质粒中扩增出1A8VLCκ基因及1A8Fd基因,并使基因两端携带合适的限制性酶切位点,将其克隆入植物表达载体pCAMBIA2301或pBIl21,构建1A8VLKCκ-pCAMBIA2301和1A8Fd-pBI121。用PCR方法改造酶切位点,继而将含有1A8Fd基因的植物表达框克隆入1A8VLCκ-pCAMBIA2301,构建获得1A8Fab-pCAMBIA2301重组质粒,并导入农杆菌GV3101。为进一步在植物中表达该抗体片段奠定了基础。1A8VLCκ and 1A8Fd gene fragments were amplified and proper restriction enzyme sites were added at each end by PCR. They were respectively cloned into pCAMBIA2301or pBI121 to construct 1A8VLCκ-pCAMBIA2301 and 1A8Fd-pBI121. The entire expression frame containing 1A8Fd gene fragment was cloned into lA8VLCκ-pCAMBIA2301 subsequently to construct 1A8Fab-pCAMBIA2301. The recombinant has been transformed into Agrobacterium tumefaciens GV3101 and laid a solid foundation for further study on expression of certain antibody fragment in plant.

关 键 词：汉坦病毒 抗体 Fab植物表达载体 

分 类 号：Q782[生物学—分子生物学]