螺旋藻多糖对衰老小鼠肺细胞凋亡与超微结构的影响  

Effects of polysaccharide from spirulina platensis on the pneumocyte apoptosis and ultrastructure in aging mice

作　　者：王德俊[1] 孙云[1] 徐峰[1] 周卫东 

机构地区：[1]扬州大学医学院组织学教研室,江苏省扬州市225001 [2]扬州大学检测中心电镜室,江苏省扬州市225009

出　　处：《中国临床康复》2005年第11期100-101,F003,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:研究螺旋藻多糖(polysaccharidefromspirulinaplatensis,PSP)对衰老模型肺细胞凋亡、超微结构的作用及作用机制。方法:实验选用2月龄健康ICR品系小鼠50只,雌雄各半。随机数字表法随机分为模型组和PSP高、中、低3个剂量组及对照组,每组10只。取2月龄小鼠在常规条件下饲养11个月,制成自然衰老模型,应用PSP20,40,80mg/kg剂量分别灌服衰老小鼠6周,借助荧光显微镜研究肺组织细胞凋亡、电子显微镜下的形态学变化。结果:衰老可诱导肺组织细胞凋亡及肺泡Ⅱ型上皮细胞超微结构的改变,PSP可明显抑制肺组织细胞凋亡,使凋亡细胞数减少,改善肺泡上皮细胞的结构与功能。PSP明显减轻衰老引起的肺细胞凋亡,PSP高剂量组对凋亡改变相应减轻,细胞凋亡率呈下降趋势且明显犤(14.2±2.24)%犦,与模型组犤(23.7±5.53)%犦比较,差异有非常显著性意义(t=4.6721,P<0.01);PSP中、低剂量组犤(16.3±3.79)%,(19.6±4.61)%犦与模型组比较,差异有显著性意义t=3.2054,P<0.01;(t=1.6219,P<0.05);PSP高中剂量与对照组犤(12.8±3.54)%犦比较,差异无显著性意义(P>0.05),PSP低剂量与对照组比较,差异有显著性意义(t=3.5193,P<0.01)。结论:螺旋藻多糖对衰老小鼠肺组织细胞的退行性变化具有改善或延缓作用。AIM: To study the effect of polysaccharide from spirulina platensis (PSP) on the pneumocyte apoptosis and ultrastructure in aging mice and its functional mechanism. METHODS: Totally 50 ICR mice, aged 2 months, of either gender, were selected and divided into model group, three dosage groups with high, medium and low PSP, and control group, 10 in each. Two month old mice were fed for 11 mouths under normal condition to establish natural aging models. The 20, 40, 80 mg/kg PSP was administrated respectively to the aging mice for 6 weeks, and the apoptosis of pulmonary histocyte and morphological changes under electron microscope were investigated with fluorescence microscope.RESULTS: Aging could induce the apoptosis of pulmonary histocyte and changes of ultrastructure of epithelial cells in pulmonary alveolus Ⅱ . PSP could significantly inhibit apoptosis of pulmonary histocyte, decrease the number of apoptosis cells, improve the structure and function of epithelial cells in pulmonary alveolus.PSP significantly decreased apoptosis of pulmonary histocyte induced by aging. Apoptosis in the PSP high dosage group was relatively lessened, and very significantly decreased [(14.2± 2.24)% ] as compared with that of model group [(23.7± 5.53)% ](t=4.672 1, P< 0.01);Apoptosis in the PSP medium and low dosage groups [(16.3 ± 3.79)% ,(19.6± 4.61)% ]were significantly different from that in the model group (t=3.205 4, P< 0.01; t=1.621 9, P< 0.05); Differences of apoptosis in the PSP high and medium dosage groups and control group [(12.8± 3.54)% ] had no significance (P >0.05); Differences of apoptosis in the PSP low dosage group and control group were significant (t=3.519 3, P< 0.01).CONCLUSION: PSP has ameliorating and postponing effects on the regressive changes of pulmonary histocyte in aging mice.

关 键 词：多糖类/药理学 肺/细胞学 肺/超微结构 

分 类 号：R56[医药卫生—呼吸系统]