补肾方药对实验性骨质疏松症大鼠垂体-甲状腺轴及其骨密度的影响(英文)  

Effect of recipe for kidney tonification on pituitary-thyroid axis and bone density in rat with experimental osteoporosis

作　　者：武密山[1] 李恩[2] 赵素芝[3] 白霞[1] 李爱英[1] 孟云辉[4] 

机构地区：[1]河北医科大学中医学院方剂教研室 [2]河北医科大学生化教研室 [3]石家庄市桥东区医院中医科 [4]河北医科大学,硕士研究生河北省石家庄市050091

出　　处：《中国临床康复》2005年第11期187-189,共3页Chinese Journal of Clinical Rehabilitation

基　　金：河北省教育厅博士基金资助课题项目(B200013);河北省科技厅博士科研资助项目(02547005D);国家自然科学基金资助项目(30472200)~~

摘　　要：背景:将补肾方药分别制成“外贴剂”和“口服剂”,根据“体表穴位-经络-内脏-靶器官”相关,分别用外贴穴位和口服两种不同的给药途径治疗骨质疏松,按照中医传统理论推测均会归入肾经”而发挥调节作“用,但实际上是否会有“靶向给药”的特异性?检测“下丘脑-垂体-靶腺”系统的激素,进一步分析肾、骨、子宫、甲状腺、睾丸等靶器官的雌激素受体和雄激素受体,观察归经与受体的相关性。“”设计:以骨质疏松大鼠为研究对象的完全随机设计,补肾方药不同给药途径对照实验研究。目的:探讨补肾方药对实验性骨质疏松垂体-甲状腺轴的影响。单位:河北医科大学中医学院。材料:实验于2000-01/2004-12在河北医科大学中西医结合基础实验室完成。选择3月龄健康SD雌性大鼠60只,体重(300±20)g。实验动物由河北省实验动物中心提供。大鼠在实验室常规喂养1周后,随机分成6组:正常对照组(10只);病理模型组(10只);补肾方药总成分按0.8g/100g体质量灌喂组10只;穴位贴剂外贴膀胱经穴位组10()(只);穴位贴剂外贴肾经穴位组(10只);穴位贴剂外贴非经非穴位组(10只)。方法:采用注射地塞米松建立骨质疏松模型,用抗骨松穴位贴剂(antagonismosteoporosisacupointpaste,AOAP)外贴穴位、非经非穴位、并与口服补肾方药比较治疗骨质疏松(OP)。BACKGROUND:In the research,the recipe for kidney tonification is prepared into “ topical form” and “ oral form” .According to the interrelationship of “ acupoints meridans and collaterals internal organs target organs” ,osteoporosis is treated by the approaches of point compress and oral application respectively.According to the traditional theory of Chinese medicine,it is to infer that the herbs bring the adjustment into play by “ being attributive to kidney meridian” , but,whether does the specialty of herbs on “ targeting medication” happen practically or not? Hormones in “ hypothalamus pituitary target gland” system is assayed to further analyze estrogen receptor and androgen receptor of target organs,such as kidney,bone, uterus,thyroid gland and testis so as to observe the relativity between “ tropism and receptor” .DESIGN:Complete random design with the objects of osteoporosis rats,and the control experimental study with kidney tonification recipe administrate with different approaches.OBJECTIVE:To probe into the effect of kidney tonification recipe on pituitary thyroid axis in experimental osteoporosis.SETTING:College of Traditional Chinese Medicine of Hebei Medical University.MATERIALS:The experiment was performed in Chinese Western integrated Basic Experiment Room of Hebei Medical University from January 2000 to December 2004.Sixty SD healthy female rats of 3 month old were employed, weighted (300± 20) g.The experimental animals were provided by Hebei Experimental Animal Center. After bred routinely for 1 week in Experimental Room, the rats were randomized into 6 groups,namely normal control(10 rats), pathological model group(10 rats),infusing group(10 rats) in which kidney tonification recipe was applied based on 0.8 g/100 g body mass,acupoint paste on bladder meridian group (10 rats), acupoint paste on kidney meridian group(10 rats) and paste on non meridian group(10 rats). METHODS:Osteoporosis model was prepared with injection of dexamethasone.Antagon

关 键 词：骨质疏松 补肾方药 垂体 甲状腺 骨密度 

分 类 号：R681.4[医药卫生—骨科学]