基质金属蛋白酶3,12基因多态性与汉族人群急性冠状动脉综合征危险性的关系(英文)  

Association of matrix metalloproteinase-3 and -12 genes polymorphism with acute coronary syndrome in Chinese Han population

作　　者：熊鹰[1] 魏晓艳[1] 杨秀兰[1] 王玲[2] 何春燕[3] 周新[4] 

机构地区：[1]武警湖北总队医院急诊科,湖北省武汉市430061 [2]华中科技大学梨园医院内科 [3]武汉大学医学院生化与分子生物学系 [4]武汉大学中南医院检验系,湖北省武汉市430071

出　　处：《中国临床康复》2005年第11期226-228,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:急性冠状动脉综合征(acutecoronarysyndrome,ACS)发生通常是由于动脉壁不稳定粥样斑块的破裂和血栓形成造成的,基质金属蛋白酶(MMPs)通过降解细胞外基质蛋白,在血管重构及斑块不稳定性中发挥重要作用。目的:探讨MMP-3和MMP-12基因启动子多态性与中国汉族人群中ACS危险性的可能关系。设计:以ACS患者为研究对象,健康人群为对照组的病例-对照研究。单位:一所大学医院的检验系和一所医学院的生化与分子生物学系。对象:研究对象为武警湖北总队医院、武汉大学中南医院和华中科技大学梨园医院2002-03/2003-12的门诊和住院患者,分为ACS组和正常对照组。ACS组103例,男73例,女30例,平均年龄(53±10)岁,包括急性心肌梗死47例、不稳定心绞痛56例;正常对照组为100例无血缘关系的健康个体(冠脉造影正常),男67例,女33例,平均年龄(52±12)岁;以上研究对象均为湖北地区汉族人,均自愿参加本研究。方法:采用多聚酶链反应-限制性片段长度多态性(PCR-RFLP)分析方法,分别检测了湖北地区汉族103例ACS患者及100例健康人的MMP-3基因-16125A/6A和MMP-12基因-82A/G多态性的基因型。主要观察指标:两组基因型和等位基因分布频率的比较。结果:ACS组MMP-3基因的5A/6A+5A/5A基因型频率及5A等位基因频率显著高于健康对照组(41.BACKGROUND:Acute coronary syndrome(ACS) are a result of the rupture of unstable atherosclerotic plaques and thrombosis.Matrix metalloproteinases(MMPs) are believed to play an important role in vascular remodeling and plaque instability by degrading the extracellular matrix.OBJECTIVE:To investigate the possible impact of polymorphisms in matrix metalloproteinase 3 and 12 genes on the risk of ACS in Chinese Han population. DESIGN:A case control study based on ACS patients as subjects and healthy population as the control group.SETTING:Department of clinical tests of a university hospital and the department of biochemistry and molecular biology of a medical collegePARTICIPANTS:A total of 103 patients with ACS( outpatients and inpatients received and hospitalized from March 2002 to December 2003 at the People's Armed Police Hubei General Hospital, Zhongnan Hospital of Wuhan University and Liyuan Hospital of Huazhong University of Science and Technology) were involved and were divided into ACS group and the normal control group.For the ACS group,there were 73 males and 30 females with an average age of(53± 10) years,including 47 cases of acute cardiac infarction and 56 cases of unstable cardiac angina.For the normal control group, there were 100 healthy subjects as controls,including 67 males and 33 females with an average age of(52± 12) years.All the subjects were Chinese Han Population in Hubei Province,and they were voluntary to participate in the study.METHODS:Polymerase chain reaction(PCR) and restriction fragment length polymorphism(RFLP) techniques were used to examine the MMP 3 1612 5A/6A and MMP 12 82 A/G gene polymorphisms respectively.MAIN OUTCOME MEASURES:The genotypes of and the allele frequencies in the patients and the individuals in the control group.RESULTS:The prevalence of 5A/6A+ 5A/5A genotype( 41.7% vs 24% ,χ 2=6.447, P< 0.05) and 5A allele (21.8% vs 13% , χ 2=4.906,P< 0.05) of MMP 3 gene were significantly more frequent in the ACS group than in the contr

关 键 词：冠状动脉疾病 金属蛋白酶类 多态现象(遗传学) 聚合酶链反应 

分 类 号：R543.3[医药卫生—心血管疾病]