血管紧张素Ⅰ转换酶基因多态性与2型糖尿病心血管并发症关联研究(英文)  

Correlation analysis between the genetic polymorphism of angiotensin I converting enzyme and the cardiovascular complications of type 2 diabetes

作　　者：武守山[1] 田喜凤[1] 

机构地区：[1]华北煤炭医学院生物科学系普通生物学教研室,河北省唐山市063000

出　　处：《中国临床康复》2005年第11期234-235,共2页Chinese Journal of Clinical Rehabilitation

基　　金：河北省科技厅科技攻关项目(022761106)~~

摘　　要：背景:糖尿病晚期并发症的发生与遗传因素有关。有报道血管紧张素Ⅰ转换酶(angiotensinⅠ-convertingenzyme,ACE)基因是糖尿病心血管并发症发生的风险因子。目的:探讨ACE基因多态性与2型糖尿病心血管并发症之间的相关性。设计:以2型糖尿病患者为研究对象的病例-对照研究。单位:一所医学院的生物科学系普通生物学教研室。对象:本研究于2001-02/2002-06在华北煤炭医学院分子生物学实验室完成。研究对象均为本医学院附属医院门诊及住院2型糖尿病患者,根据是否合并心肌梗死分为两组。心肌梗死患者组68例,其中男29例,女39例,年龄(65±3)岁;非心肌梗死组190例,其中男161例,女29例,年龄(60±1)岁。纳入标准:均符合1985年WHO2型糖尿病诊断标准,心肌梗死诊断根据MRI确诊;排除标准:资料不完整或不合作者。方法:按常规酚/氯仿法提取基因组DNA,扩增产物用20mL/L琼脂糖凝胶电泳分析,溴化乙锭染色观察结果。对不同患者组等位基因和基因型频率进行比较。主要观察指标:两组患者ACE基因的等位基因和基因型分布比较。结果:2型糖尿病心肌梗死患者64.7%与非心肌梗死患者55.0%比()()较,DD纯合子频率显著增高(41.2%vs33.2%),差异具有显著性意义(χ2=0.833,P<0.05),D等位基因频率也显著增高,差异也具有显著性意义(χ2=3.8491。BACKGROUND:The occurrence of the late complications of diabetes is correlated with genetic factors.It has been reported that angiotensin I converting enzyme(ACE) gene is considered as the risk factor of the occurrence of the diabetic cardiovascular complications.OBJECTIVE:To investigate the correlation between the polymorphism of ACE gene and the cardiovascular complications of type 2 diabetes.DESIGN:A case controlled study based on type 2 diabetic patients. SETTING:A laboratory of general biology,faculty of biology in a medical college.PARTICIPANTS:The study was conducted in the Molecular Biological Laboratory of North China Coal Medical College between February 2001 and June 2002.Type 2 diabetic patients selected from outpatient and inpatient departments of the affiliated hospital of our college were divided into two groups based on the complication of myocardial infarction.Myocardial infarction group(infarction group, n=68) included 29 males and 39 females aged of(65± 3) years old;non myocardial infarction group(non infarction group,n=190) included 161 males and 29 females aged of(60± 1) years old.Inclusion criteria:All patients were in accordance with WHO diagnostic criteria for type 2 diabetes set in 1985,and myocardial infarction was diagnosed by MRI;Exclusion criteria:Patients who had incomplete data or who were uncooperative. METHODS:Genome DNA was extracted by routine phenol/chloroform method.Amplified products were analyzed by 20 mL/L of agarose gel electrophoresis.The results were observed after ethidium bromide staining.The frequency of alleles and genotype was compared between two groups.MAIN OUTCOME MEASURES:The comparison of the distribution of ACE alleles and genotype in both groups of patients.RESULTS:DD homozygote frequency of type 2 diabetic patients complicated with myocardial infarction(64.7% ) significantly increased to 41.2% (χ 2=0.833,P< 0.05) compared with 33.2% of patients without myocardial infarction (55.0% ),while D allele frequency also significantly increased in patients w

关 键 词：2型糖尿病 心肌梗塞 肽基二肽酶A 多态现象 

分 类 号：R587.1[医药卫生—内分泌]