银杏叶提取物EGb761对TNF-α诱导HeLa细胞凋亡的影响  

Effects of Ginkgo Biloba Extract EGb761 on Apoptosis Induced by TNF-α in HeLa Cell

作　　者：黄迪南[1] 侯敢[1] 

机构地区：[1]广东医学院生物化学与分子生物学研究所,524023

出　　处：《实用癌症杂志》2005年第1期31-33,共3页The Practical Journal of Cancer

摘　　要：目的　初步研究银杏叶提取物EGb761对重组人肿瘤坏死因子- α(rhTNF- α)诱导HeLa细胞凋亡的影响。方法　采用MTT法观察EGb761对HeLa细胞生长的影响;细胞凋亡实验分5组,即空白对照组,rhTNF -α对照组(10 0 μg/L ) ,EGb761低浓度组[rhTNF- α(10 0 μg/L) +EGb761(10mg/L) ] ,EGb761中浓度组[rhTNF -α(10 0 μg/L ) +EGb761(2 0mg/L ) ] ,EGb761高浓度组[rhTNF -α(10 0 μg/L) +EGb761(4 0mg/L) ] ,采用流式细胞术和Hoechst3 3 2 5 8荧光染色检测凋亡。结果　EGb761在5 .0～80 .0mg/L终浓度范围内对HeLa细胞生长无明显影响;流式细胞术测定各组亚倍体细胞峰积分百分率(% )为:空白对照组(5 .3 5±2 .2 ) ,rhTNF- α对照组(3 7.8±6.1) ,EGb761低浓度组(19.2±3 .4) ,EGb761中浓度组(16.5±5 .7)和EGb761高浓度组(11.3±3 .9) ;荧光染色检测各组凋亡百分率(% ) :对照组(4 .2 3±2 .0 )、rhTNF α对照组(2 7.8±4.3 )、EGb761低浓度组(14 .9±3 .4)、EGb761中浓度组(12 .1±3 .7)和EGb761高浓度组(9.61±2 .3 ) ;结果显示,EGb761在10～40mg/L终浓度范围内对rhTNF α诱导的HeLa细胞凋亡均有显著的抑制作用(P <0 .0 1) ,且呈剂量依赖关系。结论　EGb761能有效抑制rhTNF- α诱导的HeLa细胞凋亡。Objective To investigate the effects of Ginkgo biloba extract EGb 761 on apoptosis of HeLa cell induced by tumor necrosis factor-α(TNF-α).Methods MTT method was used to observe the effect of EGb761 on HeLa cell growth.Flow cytometry and Hoechst 33258 fluorescent staining were used to detect the effect of EGb761 on the apoptosis of HeLa cell induced by rhTNF-α.Results The result of MTT detection showed that EGb761 had no effects on HeLa cell growth within 5.0～80 mg/L final concentration.The result of flow cytometry and Hoechst 33258 fluorescent staining exhibited that EGb761 with 10～40 mg/L final concentration had notable inhibition but not absolute inhibition on the apoptosis of HeLa cell induced by rhTNF-α(P<0.01) and it showed dose-dependence.Conclusion The apoptosis of HeLa cell induced by rhTNF-α can be efficiently inhibited by EGb761.

关 键 词：HELA细胞凋亡 EGB761 银杏叶提取物 Hoechst33258 HELA细胞生长 重组人肿瘤坏死因子α RHTNF 荧光染色检测 流式细胞术 剂量依赖关系 浓度范围 对照组 低浓度 MTT法 抑制作用 百分率 步研究 体细胞 

分 类 号：R285[医药卫生—中药学] R743.31[医药卫生—中医学]