1．06μm激光血管成形术后动脉平滑肌细胞中的癌基因表达  

Oncogene Expression in Arterial Smooth Muscle Cells After 1.06μm Laser Angioplasty

作　　者：陈明哲[1] 陈大新[1] 陈凤荣[1] 

机构地区：[1]北京医科大学第三医院心内科

出　　处：《中国激光医学杂志》1994年第1期5-8,共4页Chinese Journal of Laser Medicine & Surgery

基　　金：国家教委基金

摘　　要：通过建立兔腹主动脉粥样硬化性狭窄模型进行１．０６μｍ激光血管成形术（ＬＡ），应用系列癌基因片段与地高辛－ｄＵＴＰ随机插入标记，进行血管组织的原位杂交。发现：（１）ＬＡ后血管壁可见ｃ－ｆｏｓ、ｃ－ｊｕｎ、Ｃ－ｍｙｃ和Ｎ－ｒａｓ等癌基因表达；（２）癌基因表达多位于血管斑块中的ＳＭＣ；（３）ｃ－ｆｏｓ、ｃ－ｊｕｎ表达迅速而短暂，ｃ－ｍｙｃ和Ｎ－ｒａｓ表达较迟，持续时间较长。结论：激光损伤可诱导动脉ＳＭＣ中的某些癌基因表达。μm laser angioplasty(LA)was carried out on experimentally developed artherosclerotic stenosis of ab-dominal aorta in rabbits.The vascular tissues after AL were hybridized with probes of oncogenes labeled by ran-domly inserted digoxin-dUTP.The results showed that:(1)There was expression of oncogenes c-fos,c-jun,c-myc and N-ras in vascular wall after LA.(2)The oncogen expression was mostly positive in the intima.(3)c-fos and c-jun were expressed instantly and temporarily.while expression of c-myc and N-ras occurred later andpersisted for a longer time.It was concluded that expression of certain oncogenes in arterial smooth muscle cellscould be induced by laser irradiation.

关 键 词：激光手术 血管成形术 癌基因 SMC 

分 类 号：R654.3[医药卫生—外科学]