油酸诱导SW872前脂肪细胞分化及分化过程中促酰化蛋白的分泌  

Oleic acid inducing SW872 preadipocytes differentiation and the secretion of acylation stimulating protein during the course of differentiation

作　　者：张龙江[1] 卢慧玲[1] 王宏伟[1] 夏治[1] 林汉华[1] 

机构地区：[1]华中科技大学同济医学院同济医院儿科,湖北武汉430030

出　　处：《基础医学与临床》2005年第4期354-357,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(30170442)

摘　　要：目的探讨油酸对SW872前脂肪细胞的诱导分化作用及SW872细胞分化过程中促酰化蛋白的分泌。方法体外培养SW872细胞,0.6mmol/L油酸作为诱导分化剂刺激SW872前脂肪细胞,油红O染色观察细胞形态的变化及脂滴的聚集,化学比色法测定甘油三酯的总量,ELISA方法测定培养上清中促酰化蛋白的含量。结果SW872细胞分化之前呈成纤维细胞样,胞内无脂滴,甘油三酯的含量较低;加入0.6mmol/L油酸诱导分化刺激72h时,SW872细胞已经完全分化,胞浆中脂滴明显增多,油红O染色可见胞浆中丰富的脂肪染色,甘油三酯的含量是诱导分化前的14倍(P<0.01)。诱导分化之前,SW872细胞分泌ASP的量非常低,几乎不能检测出;诱导分化72h,ASP的含量增加到1.12ng/mg细胞总蛋白质。结论油酸能诱导SW872前脂肪细胞分化为成熟的脂肪细胞;ASP的分泌与脂肪细胞的分化程度密切相关。Objective To study the differentiation effect of oleic acid on SW872 preadipocytes and the secretion of acylation stimulating protein by SW872 cells. [WT10.HZ]Methods[WT] SW872 preadipocytes were cultured in vitro and induced to differentiate by (0.6 mmol/L) oleic acid.During the progress of differentiation, morphologic changes of SW872 cells were observed, Triglyceride(TG) mass was detected by chemical colorimetry methods,the levels of Acylation stimulating protein was assayed by ELISA. [WT10.HZ]Results[WT] Before induction, SW872 cells were fibroblastic, there was no fat droplets in cytoplasm, the levels of TG mass were lower.(72 h) after (0.6 mmol/L) oleic acid addition,SW872 preadipocytes differentiated into mature adipocytes with lots of fat droplets in the cells. The levels of TG mass were increased up to 14.0-fold(P<0.01). Before induction,the level of ASP was too low to be detected.Seventy-two h's after oleic acid addition, the levels of ASP increased to (1.12 ng/mg) total cell protein. Conclusion[WT] These observations suggested that:SW872 preadipocytes could be induced to differentiate into typical mature adipocytes by (0.6 mmol/L) oleic acid. ASP production was associated with the differentiation of fat cells.

关 键 词：脂肪细胞分化 促酰化蛋白 分化过程 油酸诱导 0.6mmol/L ELISA方法 前脂肪细胞 诱导分化作用 甘油三酯 诱导分化剂 比色法测定 ASP 体外培养 细胞形态 染色观察 培养上清 脂肪染色 细胞分泌 总蛋白质 mg细胞 分化程度 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]