大鼠原代肾小管上皮细胞上IL-18Rα、β链的表达  

Expressions of IL-18Rα and IL-18Rβ on rat primary renal tubular epithelial cells

作　　者：杜胜华[1] 刘华锋[1] 唐德燊[1] 陈孝文[1] 

机构地区：[1]广东医学院肾脏病研究所,广东湛江524023

出　　处：《细胞与分子免疫学杂志》2005年第3期293-295,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：广东医学院博士启动基金资助项目(No.2002GDMC102504)

摘　　要：目的:检测培养的大鼠原代肾小管上皮细胞(renal tubularepithelialcells,RTECs)是否表达IL-18Rα和IL-18RβmRNA。方法:以肾小管节段贴块法进行原代RTECs的培养。用免疫细胞化学染色法鉴定细胞的类型,用RT-PCR检测RTECs中IL-18RαmRNA及IL-18RβmRNA的表达。结果:免疫细胞化学染色法证实,原代培养的细胞均为RTECs,并表达IL-18RαmRNA和IL18RβmRNA。结论:在RTECs中有IL-18RαmRNA和IL-18RβmRNA的表达,为探讨IL-18R在肾间质纤维化中提供了实验依据的作用。AIM: To detect the expressions of the IL-18Rα mRNA and IL-18Rβ mRNA in primary rat renal tubular epithelial cells (RTECs). METHODS: The culture of primary RTECs was performed by renal tubular segment sticking mass method. The cellular type was identified by immunocytochemical staining. The IL-18Rα mRNA and IL-18Rβ (mRNA) expressions in RTECs were detected by RT-PCR. RESULTS: The result of immunocytochemical staining proved that the cultured cells were RTECs, IL-18Rα mRNA and IL-18Rβ mRNA were detected in RTECs. CONCLUSION: IL-18Rα mRNA and IL-18Rβ mRNA are expressed in RTECs, which provide the experimental basis for exploring IL-18R role in renal interstitial fibrosis.

关 键 词：肾小管上皮细胞 IL-18Rα IL-18Rβ 表达 大鼠 

分 类 号：R392.12[医药卫生—免疫学]