MMP-2、9在TRAP^+单个核和多核细胞的表达及其在关节软骨损伤中的作用  

Expression of MMP-2 and MMP-9 in the tartrate-resistant acid phosphatase (TRAP)-positive mononuclear and multinucleated cells is correlated with articular cartilage damage

作　　者：赵雯[1] 朱平[1] 王彦宏[1] 

机构地区：[1]第四军医大学西京医院临床免疫科,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2005年第3期349-352,共4页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的:观察在胶原诱导的C57BL/6小鼠类风湿性关节炎(CIA)模型中,基质金属蛋白酶-2、9在耐酒石酸盐酸性磷酸酶(TRAP)阳性的单个核及多核细胞中的表达及其在关节软骨损伤中的作用。方法:注射鸡II型胶原免疫C57BL/6小鼠建立CIA模型。在CIA小鼠滑膜及滑膜软骨交界处,用酶组织化学及免疫组化染色分别检测TRAP+细胞的数量及细胞质内MMP的表达与软骨损伤的关系。结果:酶组织化学法检测表明TRAP+单个核及多核细胞增多的数量与CIA小鼠软骨的破坏程度呈正相关(rs=0.903,P<0.01)。免疫组化染色显示TRAP+细胞胞质内有MMP-2、9的表达,且表达的增多与CIA小鼠软骨的破坏程度呈正相关(rs=0.954,P<0.01)。结论:在CIA小鼠病变关节增生的滑膜组织及浸润到软骨表面的滑膜组织中的TRAP+单个核及多核细胞可表达MMP-2、9,表达的增多与软骨的破坏呈正相关,这些细胞在关节软骨的损伤中起到重要的作用。AIM: To examine the role of matrix metalloproteinases(MMP) expressed by the tartrate-resistant acid phosphatase (TRAP)-positive mononuclear and multinucleated cells in articular cartilage damage. METHODS: C57BL/6 mice were immunized by injection of chicken type II(CII) collagen to construct the collagen-induced rheumatoid arthritis(CIA) model. The presence of TRAP positive cells in the synovial tissue of CIA mice was examined by enzyme histochemistry and expression of MMP-2、 9 was assessed in TRAP positive cells by immunohistochemistry. RESULTS: Expression of MMP-2 (gelatinase A) and MMP-9 (gelatinase B) was detected in TRAP positive mononuclear and multinucleated cell. Quantity of TRAP positive cells and the destruction of articular cartilage had a positive correlation (r_s=0.903, P<0.01); Expression of MMP-2 and MMP-9 in TRAP positive cells was also correlated significantly with the destruction of articular cartilage (r_s=0.954, P<0.01). CONCLUSION: This study suggests that MMP-2 and MMP-9 expression by TRAP positive mononuclear and multinucleated cells are involved in articular cartilage destruction in CIA.

关 键 词：胶原诱导关节炎 耐酒石酸盐酸性磷酸酶 基质金属蛋白酶 

分 类 号：R593.22[医药卫生—内科学]