热诱导大鼠睾丸生精上皮多核细胞的出现和变化  

Appearance and Change of Heat Induced Multinucleate Cells in Seminiferous Epithelium in Rat Testis

作　　者：许多[1] 朱伟杰[2] 王自能[1] 秦达念[3] 

机构地区：[1]暨南大学附属第一医院妇产科,广州510632 [2]暨南大学生殖免疫研究中心,广州510632 [3]汕头大学医学院生理教研室,汕头515031

出　　处：《生殖与避孕》2005年第4期201-204,共4页Reproduction and Contraception

摘　　要：目的:研究大鼠睾丸局部热作用后生精上皮多核细胞的出现和变化。方法:雄性Sprague-Dawley大鼠50只,随机分热处理组和对照组,热处理组按热处理后1d、3d、6d、10d和25d随机分成5个亚组(n=6),每亚组相应设立对照组(n=4)。应用HE染色、原位末端标记法(TUNEL)和免疫组织化学方法检测大鼠睾丸局部受热后1d、3d、6d、10d和25d生精上皮组织学结构变化,多核细胞凋亡情况以及Fas和FasL在多核细胞中的表达情况。结果:热作用后生精上皮出现多核细胞,以1d、3d组最多见,并且多核细胞存在凋亡现象, Fas和FasL在多核细胞中亦可见表达。结论:大鼠睾丸局部热作用诱导生精上皮多核细胞出现,且随热作用后时间不同而变化。多核细胞在生精上皮热损伤中可能具有独特的生物学意义。Objectives: To investigate the appearance and change of multinucleate cells in the seminiferousepithelium induced by local heating of the rat testis. Methods: Fifty adult male Sprague-Dawley rats were randomlydivided into experimental group and control group. According to day 1, 3, 6, 10 and 25 after local testicular heating,each group was divided into 5 subgroups: experimental subgroup (n=6) and control subgroup (n=4). The histologyof testis and the apoptosis and Fas /FasL expression of multinucleate cells were detected on day 1, 3, 6, 10 and 25after heat-exposure by HE staining, TUNEL (TdT-mediated dUTP-X nick end labeling) method andimmunohistochemistry, respectively. Results: After been heated, multinucleate cells appeared in seminiferousepithelium and were the most in groups of 1 d and 3 d. The apoptosis of multinucleate cells and the expression ofFas /FasL located in multinucleate cells were found. Conclusions: Local heating of the rat tastis could induce theappearance of multinucleate cells in the seminiferous epithelium. And the number of multinucleate cells changedalong with the time after heating. Multinucleate cells may have special biological meaning on heat injury ofseminiferous epithelium.

关 键 词：多核细胞 热作用 睾丸 大鼠 

分 类 号：Q952[生物学—动物学]