五个抑癌印迹基因在人类卵子及种植前胚胎的表达  

Expression of Five Imprinted Tumor Suppressor Genes in Human Oocytes and Preimplantation Embryos

作　　者：沈文洁[1] 孔令红[1] 陈士岭[1] 李红[1] 邢福祺[1] 

机构地区：[1]第一军医大学南方医院生殖中心

出　　处：《生殖与避孕》2005年第4期210-214,共5页Reproduction and Contraception

基　　金：广东省科技计划项目(2003c34212)

摘　　要：目的:检测5个抑癌印迹基因在人类卵子和种植前胚胎的正常表达,完善印迹基因在人类种植前胚胎的表达图谱。方法:应用巢式RT-PCR技术检测印迹基因ARHI、LOT1、P57KIP2、Peg3、TSSC3在人类卵子和种植前胚胎中的表达。结果:卵子和各期种植前胚胎、囊胚中均存在LOT1、P57kip2转录;除4-细胞胚胎外,卵子和其余各期胚胎均可测得ARHI表达; Peg3表达于4-细胞、8-细胞胚胎和囊胚;TSSC3于卵子及各期种植前胚胎中均未表达。结论:除TSSC3外的4个抑癌印迹基因均表达于种植前胚胎,为探讨印迹基因与ART及肿瘤的关系提供理论依据。Objective: To investigate the expression of five imprinted tumor suppressor genes in humanoocytes and preimplantation embryos, and identify more genes expressed in preimplantation embryos. Methods: Usingnested RT-PCR to analyze the expression of ARHI,LOT1,P57KIP2,Peg3, and TSSC3 in human oocytes and preim-plantation embryos. Results: Transcripts of P57kip2 and LOT1 were detected in human oocytes and at all stages ofpreimplantation. ARHI was expressed in oocytes and 2-, 8-cell embryos and blastocyst. Peg3 mRNA existed in 4-,8-cell embryos and blastcyst.Transcripts of TSSC3 could not be detected in human oocytes and preimplantationembryos. Conclusion: Except for TSSC3,transcripts of four other imprinted genes were detected in human preim-plantation development, which provides basis for understanding the correlation among imprinted genes, ART andtumor.

关 键 词：印迹基因 种植前胚胎 辅助生育技术 抑癌基因 

分 类 号：Q987[生物学—遗传学]