应激后大鼠下丘脑磷酸化p38MAPK的变化及电针足三里穴的调节作用  

The studies on phosphorylated-p38 MAPK in hypothalamus from restrain stress rat and the regulation of p38 MAPK by electroacupuncture

作　　者：万顺伦[1] 孙锦平[1] 李召峰[2] 

机构地区：[1]解放军总医院神经信息中心 [2]第二军医大学生理学教研室

出　　处：《中华物理医学与康复杂志》2005年第4期202-204,共3页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的观察应激对大鼠下丘脑磷酸化p38MAPK的影响及电针足三里穴的调节作用. 方法将28只Wistar大鼠随机分为对照组、束缚应激组和束缚应激+电针组,后2组再根据束缚解除后及电针足三里穴位治疗后不同时间点分别分为3个亚组.电针治疗采用频率为5～12 Hz的疏密波,治疗总时间30 min.于相应时间点处死各组大鼠,采用Western印迹杂交法观察其下丘脑内磷酸化p38MAPK的变化. 结果束缚应激组大鼠下丘脑内p38MAPK磷酸化水平明显高于对照组,且在束缚应激解除后3 h仍保持在较高水平;应激大鼠经电针足三里穴处理后,下丘脑内磷酸化p38MAPK与束缚应激组相比明显回落,这种调节作用可持续到电针治疗结束后3 h. 结论束缚应激能引起下丘脑应激信号转导系统中p38MAPK的磷酸化,这可能是应激活动过程中的重要事件之一.电针足三里穴对下丘脑内磷酸化p38MAPK的调节作用可能与下丘脑-垂体-肾上腺轴的活动有关.Objective To observe the effects of restrain stress on the phosphorylation of p38 mitogen-activated protein kinase ~MAPK) in rat hypothalamus and the regulation effects of electroacupuncture on the phosphorylated-p38 MAPK. Methods Twenty-eight rats were randomly divided into a control group, a restrain stress group and a restrain stress plus electroacupuncture group. The Western blot was employed to observe the changes of phosphorylated-p38MAPK in the three groups. Results Western blot analysis demonstrated a higher level of phosphorylation of p38 MAPK in the hypothalamus from restrain stress group than that from control. The higher level of phosphorylated-p38 MAPK could last for 3 hours after stress was relieved. The level of phosphorylated-p38MAPK could be decreased to some extent when the Zusanli acupoint of the restrained rat was acupunctured. The effects of electroacupuncture of Zusanli on phosphorylated-p38MAPK in hypothalamus could still be retained although the electroacupuncture was ended for 3 hours. Conclusion The results suggested that restrain stress can lead to a higher level of phosphorylation of p38 MAPK in hypothalamus, which might be an important event in the response of hypothalamic-pitutary-adrenal ~HPA) axis. The mechanism underlying the regulation of HPA by electroacupuncture might be (related) to the regulation of phosphorylated-p38 MAPK in hypothalamus.

关 键 词：p38MAPK 调节作用 下丘脑-垂体-肾上腺轴 WESTERN印迹 Wistar大鼠 束缚应激 信号转导系统 电针治疗 足三里穴位 磷酸化水平 不同时间 应激大鼠 对照组 脑内 治疗后 疏密波 杂交法 高水平 解除 活动 

分 类 号：R245[医药卫生—针灸推拿学]