川芎嗪对小鼠骨髓基质细胞内皮抑素表达的影响  

Effects of ligustrazine on expression of endostatin in syngenic bone marrow-transplated mice

作　　者：吴宁[1] 孙汉英[1] 刘文励[1] 何莉[1] 孟凡凯[1] 李春蕊[1] 刘丹[1] 付丽[1] 罗琳[1] 徐惠珍[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院血液科,武汉430030

出　　处：《中华放射医学与防护杂志》2005年第2期121-123,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目(39870926)

摘　　要：目的探讨同基因骨髓移植(BMT)小鼠骨髓基质细胞内皮抑素(endostatin,ES)的表达及川芎嗪对其影响。方法取健康BALBc小鼠,随机分为3组正常组(不做处理),骨髓移植对照组(BMT组)和骨髓移植川芎嗪治疗组(川芎嗪组)。BMT组和川芎嗪组分别胃饲生理盐水02ml只和川芎嗪注射液每次2mg只,2次d。在BMT后第7、14、21、28天处死小鼠,采用免疫组化、Westernblot方法检测骨髓基质细胞中ES蛋白表达水平,RTPCR检测骨髓基质细胞中ESmRNA表达水平。结果BMT后第7、14、21、28天川芎嗪组和BMT组ESmRNA和蛋白表达水平均显著低于正常组(P<005或P<001),随着时间延长其表达逐渐增高。BMT后第14、21、28天川芎嗪组ES表达水平始终低于BMT组(P<005);但骨髓有核细胞计数高于BMT组(P<005或P<001)。结论川芎嗪能反馈调节BMT小鼠骨髓基质细胞新血管生成抑制因子的表达,促进骨髓微环境的修复,加速移植后骨髓的造血重建。Objective To explore the effect of ligustrazine on expression of endostatin in bone marrow stromal cells of mice after syngenic bone marrow transplatation(BMT). Methods The mice were randomly divided into 3 group:normal group (without any treatment),BMT control group and ligustrazine-treated groups.A BMT mouse model was established.The mice in the BMT control group and the ligustrazine-treated group were orally given 0.2 ml saline and 2 mg ligustrazine,respectively,twice a day.On days 7,14,21,28 after BMT,mice were sacrificed.Bone marrow nucleated cells (BMNCs) were counted and the expression of endostatin was assayed by immunohistochemistry,Western blot and RT-PCR analysis respectively. Results On the days 7,14,21,28 d after BMT,expression of endostatin in bon marrow stromal cells was significantly lower than that in the normal group(P<0.05 or P<0.01).In the ligustrazine-treated group,the number of BMNCs on the days 7,14,21,28 after BMT was higher than that in the BMT control group (P<0.05 or P<0.01).However the expression of endostatin in the former was lower than that in the BMT control group (P<0.05). Conclusion Ligustrazine can inhibit endostatin expression in bone marrow stromal cells after syngenic BMT in mice,which may be related to the ligustrazine′s accelerating hematopoietic reconstitution in mice after BMT.;

关 键 词：骨髓基质细胞 内皮抑素 BALB/c小鼠 RT-PCR检测 骨髓有核细胞计数 血管生成抑制因子 同基因骨髓移植 Western 川芎嗪注射液 蛋白表达水平 mRNA表达 BMT 0.05 骨髓微环境 生理盐水 免疫组化 blot 时间延长 反馈调节 造血重建 

分 类 号：R285[医药卫生—中药学]