血管内皮生长因子转染放疗组织的研究  

Research on revascularization of irradiated tissue by VEGF gene transfection

作　　者：于大海[1] 王代友[1] 

机构地区：[1]广西医科大学附属口腔医院口腔颌面外科,南宁530021

出　　处：《中华放射医学与防护杂志》2005年第2期132-134,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目(30060083)

摘　　要：目的用血管内皮生长因子基因转染的方法恢复放疗损伤区组织血管生成能力。方法以60Coγ射线照射大鼠右后肢,每次每只大鼠吸收剂量为2Gy,总吸收剂量为30Gy。照射结束后第1天,将重组质粒pcDNA3VEGF165转染大鼠右后肢照射区肌肉组织细胞,每次每只大鼠转染质粒200μg,共治疗2次,间隔2周,治疗结束后行VEGF165mRNA、VEGF蛋白、血管染色体视学图像分析及血管超微结构观察等检查。结果经重组质粒pcDNA3VEGF165转染后,照射区组织VEGF165mRNA及VEGF蛋白均明显增高,微血管密度、平均截面积、平均管径均达到正常组织水平,血管超微结构基本恢复正常。结论VEGF基因转染不仅有效地修复了组织血管放射损伤,而且还能恢复其血管生成能力,为临床应用提供了实验基础。Objective To resume the ability of revascularization of the irradiated tissue by VEGF gene transfection. Methods Each one of the mice was irradiated for 2 Gy every time and 30 Gy in total on the right leg. The irradiated muscular tissues of animal models were transfected with 200 μg pcDNA_3/VEGF_~165 per animal on the right leg and another 200 μg two weeks later. After the gene therapy we determined VEGF_~165 mRNA ,VEGF protein, vessel density, average diameter of vessels and average sectional aera. We also observed ultrastructure of the vessels at the same time. Results VEGF_~165 mRNA and VEGF protein were highly expressed in the irradiated tissue. Vessel density, average diameter and average sectional aera were also not different from those on the left leg, and the configuration and ultrastructure of vessels returned to normal. Conclusion VEGF gene therapy can resume the ability of revascularization of irradiated tissue, which provides the bases for its clinical application.;

关 键 词：血管内皮生长因子 VEGF165 VEGF蛋白 血管超微结构 生成能力 基因转染 吸收剂量 重组质粒 Γ射线照射 超微结构观 微血管密度 放疗损伤 组织细胞 mRNA 正常组织 放射损伤 临床应用 照射区 大鼠 恢复 分析及 体视学 截面积 

分 类 号：R818[医药卫生—放射医学]