高功率微波辐照后大鼠肺脏形态学改变  

Morphological changes of rat lung after exposure to high power microwave (HPM)

作　　者：黄小军[1] 杨家骥[1] 王春梅[1] 黄晓峰[1] 李珍[1] 于华[1] 陈丹[1] 

机构地区：[1]第四军医大学基础部电子显微镜中心,西安710032

出　　处：《中华放射医学与防护杂志》2005年第2期178-179,共2页Chinese Journal of Radiological Medicine and Protection

基　　金：军队"863"课题资助项目

摘　　要：目的观察HPM辐照后SD大鼠肺脏形态学的变化情况。方法辐照后不同时间点活杀SD大鼠取肺脏,分别用3%戊二醛以及10%福尔马林固定,用常规光镜以及扫描电镜观察大鼠肺脏形态学的变化。结果HPM辐照SD大鼠后6h,肺泡上皮细胞出现明显变性,可见肺泡上皮细胞脱落。肺泡隔断裂,形成肺大泡。辐照后24h,肺上皮细胞变性严重,肺泡隔断裂,变薄,出现广泛的肺大泡。间质水肿,炎细胞浸润。辐照后48h,肺内小静脉淤血。辐照后7d,肺泡隔局灶性恢复。结论HPM辐照可造成肺组织明显损伤,损伤具有时相性和可逆性。Objective To investigate the morphological changes of rat lung exposed to high power microwave(HPM). Methods Scanning electron microscopy and light microscopy were employed to investigate the morphological changes of rat lung at different time points after exposed to HPM. Results Obvious degeneration of alveolar epithelium and exfoliation of type I alveolar cells were found at 6 h after exposure.At ~24 h after exposure, severely degenerated type I alveolar cells,disruption of alveoalr septum,interstial edema and infiltration of inflammatory cells in lung were detected.At 48 h after exposure,mild degeneration of alveolar epithelium was still found.Alveolar epithelium recovered basically d 7 after exposure. Conclusion Exposure to HPM results in obvious changes of pneumocytes and these damages recover slowly.;

关 键 词：高功率微波辐照 形态学改变 肺脏 SD大鼠 福尔马林固定 扫描电镜观察 肺泡上皮细胞 炎细胞浸润 HPM 变化情况 不同时间 细胞脱落 细胞变性 间质水肿 静脉淤血 肺大泡 戊二醛 24h 局灶性 肺组织 可逆性 时相性 出现 断裂 

分 类 号：R594[医药卫生—内科学]