抗蓝氏贾第鞭毛虫单克隆抗体的制备和特性研究  

PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST GIARDIA LAMBLIA

作　　者：贾克东[1] 朱育光 李桂萍[1] 温培娥[1] 

机构地区：[1]山东省寄生虫病防治研究所,济宁272133

出　　处：《中国寄生虫病防治杂志》1994年第1期42-45,共4页Chinese Journal of Parasitic Disease Control

摘　　要：用体外培养的四川株蓝氏贾第鞭毛虫滋养休免疫BAIB/c小鼠,以免疫脾细胞与SP2/O骨髓瘤细胞融合,共获得3个分泌抗蓝氏贾第鞭毛虫单克隆抗体的杂交瘤细胞株。这些McAb经鉴定均属lgG_1亚类。选取活性最强的1E_2株McAb对不同贾第虫株进行抗原定位的测定,由IFA显示,该McAb与四川株和山东株滋养休的后1/3表面呈现荧光反应,但与澳大利亚株滋养体表面不显荧光,表明国内株与国外澳大利亚株滋养体表膜抗原存在株间差异。Three cell lines steadily secreting specific antibodies were obtained by fusion of SP2/0 myeloma cells with splenic lymphocytes from BALB/c mice immunized with axenic cultivated trophozoites (Sichuan isolate) in vitro. Hybridoma supernatants were screened for McAb by IFA.Three McAbs of IgG1 isotypes designated 1E2,4E7,1GS were obtained, of them 1E2 McAb was the most active in Dot-ELISA. The highest positive tiler of 1E2 cell supernatant was 1 : 1000 in Dot-ELISA and 1 : 3200 in ELISA. Giardia lamblia trophozoite and cyst antigens at the concentration of as low as 72. 8ng/ml and 138ng/ml respectively were detected at the titer 1 : 200 1E2 supernatant by ELISA,but reactions with both of Clonorchis sinensis and E. coli antigens were negative.Its target antigen located on the posterior surface of trophozoites in Shandong and Sichuan isolates and on entire surface of cysts found from stool of Shandong patients,but not on tropho-zoites surface from Australia isolate. Based on the assays with 1E2 McAb,it shows that Giardia lamblia of domestic isolates (Shandong and Sichuan)possess different surface antigen from that of foreign isolate (Australia),and may be they are biologically different.

关 键 词：单克隆抗体 制备 贾第虫 鞭毛纲 

分 类 号：R392－33[医药卫生—免疫学]