新型肽-DNA复合颗粒性疫苗的制备方法  

Preparation of a novel particular peptide-DNA dual vaccine

作　　者：唐艳[1] 吴玉章[1] 刘宏利[1] 赵建平[1] 倪兵[1] 贾正才[1] 周伟[1] 邹丽云[1] 

机构地区：[1]第三军医大学基础部全军免疫学研究所,重庆400038

出　　处：《免疫学杂志》2005年第3期229-231,235,共4页Immunological Journal

基　　金：国家重点基础研究计划(2001CB510001);国家自然科学基金(30300315)资助项目

摘　　要：目的研究、优化新型颗粒性肽-DNA复合疫苗的制备方法。方法以DNA沉淀试验、DNA阻滞试验、DNaseⅠ保护试验和电镜分析对该疫苗颗粒的制备参数进行优化。结果在NaCl浓度为87.5mmol/L,电荷比为2的制备条件下,该疫苗形成直径为20nm的均一颗粒,并可有效介导DNA疫苗所携带基因的表达。结论该研究为颗粒性肽-DNA复合疫苗获得良好的免疫学效应奠定了基础。Objective To study and optimize the technique of preparing the particular peptide-DNA dual vaccine. Methods The preparing parameters were optimised by assays of DNA precipitation, DNA gel retardation, DNase I protection, and electron transmission microscope. Results The dual vaccine was condensed into homogeneous particles with a diameter of about 20 nm at charge ratio of 2 in the 87.5 mmol/L NaCl concentration, and which could effectively mediate expression of the genes carried by vaccine particles. Conclusion Our study provides a good foundation for the preparation of particular peptide-DNA vaccine with favorable immunogenicity.

关 键 词：基因转染 阳离子肽 疫苗制备 多聚赖氨酸 

分 类 号：R392[医药卫生—免疫学]