含HPV16 E7的病毒样颗粒诱导小鼠产生特异性CTL反应  

CTL Response Induction in Mice Immunised with Chimeric BPVL1/HPV16 E7 Virus-Like Particles

作　　者：程浩[1] 叶俊[1] 周健[2] 

机构地区：[1]浙江大学医学院附属邵逸夫医院皮肤科,杭州310016 [2]澳大利亚昆士兰大学免疫和癌症研究中心

出　　处：《中华皮肤科杂志》2005年第5期291-293,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金资助项目(30271190)

摘　　要：目的探讨含BPVL1/HPV16E7嵌合型乳头瘤病毒样颗粒(VLPs)在动物小鼠体内的免疫学特性。方法将HPV16E7基因分为(a)、(b)、(c)3段,与BPVL1连接后表达的BPVL1/HPV16E7(a)、(b)、(c)嵌合型VLP分别免疫小鼠C57BL/6J,对其淋巴结淋巴细胞进行细胞毒性T淋巴细胞反应(CTL)分析。结果接受嵌合型VLPBPVL1/HPV16E7(b)免疫的小鼠淋巴结淋巴细胞可以引发很强的对C-2细胞(HPV16E7aa47-59转染的EL-4细胞)的特异性CTL反应,但不能对EL-4细胞产生细胞毒性作用。结论含BPVL1/HPV16E7嵌合型VLP作为抗原输送系统致敏小鼠T淋巴细胞并引发抗原特异性CTL反应,可能为HPV疫苗的设计提供一个新手段。Objective To study immunological property of chimeric BPVL1 / HPV16 E7 virus-like particles (VLPs) in mice. Methods HPV16 E7 gene was cut into three fragments, ligated to BPVL1 sequence, respectively, and then expressed as chimeric VLPs. The ability of the chimeric VLPs to stimulate in vivo cytotoxic response was analysed with lymphocytes taken from mice C57BL/6J lymph nodes. Results A strong CTL response against C-2 cells (HPV16 E7 aa47-59 transfected EL-4 cell) was induced in mice immunised with chimeric VLPs BPVL1 / HPV16 E7(b). Furthermore, no CTL response was detected against EL-4 cells in immunized mice. Conclusions Chimeric BPVL1/HPV16 E7 VLPs, serving as antigens, can activate mouse T lymphocytes and elicit a strong antigen-specific CTL response. Chimeric BPVL1/HPV16 E7 VLPs could be used as an efficient antigen delivery system, and might provide a novel strategy for HPV16 vaccine design.

关 键 词：特异性CTL反应 病毒样颗粒 淋巴结淋巴细胞 C57BL/6J EL-4细胞 HPV16E7 T淋巴细胞反应 细胞毒性作用 免疫学特性 HPV疫苗 嵌合型 免疫小鼠 致敏小鼠 输送系统 VLP 乳头瘤 基因分 抗原 

分 类 号：R392[医药卫生—免疫学]